Detalhes bibliográficos
| Ano de defesa: |
2023 |
| Autor(a) principal: |
Ramos , Darcieli Lima |
| Orientador(a): |
Rech, Virginia Cielo |
| Banca de defesa: |
Gutierres , Jessié Martins,
Berlese , Daiane Bolzan,
Fernandes, Liana da Silva,
Silva , William Leonardo da |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1254
|
Resumo: |
Exhaustive physical exercise can cause damage to muscle tissue through mechanical and metabolic processes such as apoptosis resulting from increased oxidative stress. Several studies are being carried out to determine whether the use of antioxidants can prevent or reverse the damage caused by exhaustive physical exercise, thus improving the post exercise recovery process and reducing pain and fatigue resulting from the increase in reactive species. Some of these studies highlight quercetin, due to its antioxidant and anti-inflammatory capacity. Among its diverse functions, quercetin can increase the activity of endogenous antioxidant enzymes. Furthermore, it acts by inhibiting the action of xanthine oxidase, the main source of reactive oxygen species during intense short term physical exercise, which helps to mitigate oxidative stress and the inflammatory response. However, quercetin has low aqueous solubility and low bioavailability, which reducesits absorption by the body. One way to improve its effectiveness isto incorporate it into a nanostructured lipid carrier. In this study, the objective was to evaluate the impact of the nanostructured lipid carrier containing quercetin on biochemical markers in the gastrocnemius muscle of rats subjected to exhaustive swimming. To achieve this, in this experimental study the animals were distributed into 6 groups: Sedentary; Sedentary with Diclofenac (10 mg/kg); Sedentary with Nanocarrier (0.55 mg/mL); Exhausting Swimming; Exhaustive Swimming with Nanocarrier (0.55 mg/mL); Exhaustive Swimming with Diclofenac (10 mg/kg). The levels of Dichlorofluorescein, carbonyls, Species reactive to thiobarbituric acid, histology and interleukins were measured, as well as the enzymatic activities of Sodium Potassium ATPase, Adenylate kinase, Pyruvate kinase, Creatine kinase, isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, muscle malate dehydrogenase gastrocnemius of all experimental groups. Our results highlight the increase in the activity of the Krebs cycle enzymes, isocitrate dehydrogenase, in the exhaustive swimming + diclofenac group, while the total Sodium Potassium-ATPase had its activity increased in the exhaustive swimming + nanocarrier group. Mitochondrial creatine kinase activity was reduced in the sedentary and swimming groups by both diclofenac and the nanocarrier. The cytosolic fraction was reduced by the nanocarrier in both the sedentary and exhaustive swimming groups. Adenylate activity was also reduced in the treated groups. It can be observed that Exhaustive Swimming per se reduces the levels of Dichlorofluorescein and Species reactive to thiobarbituric acid. The interleukins evaluated were not affected by the treatments. It is concluded that diclofenac and the nanocarrier showed similar behavior in relation to energy metabolism in the gastrocnemius muscle, probably due to their antioxidant effect of diclofenac and quercetin, as they reduced levels of Dichlorofluorescein and species reactive to thiobarbituric acid. Thus, the quercetin nanocarrier proved to be as effective as a usual anti-inflammatory drug. |
