Detalhes bibliográficos
| Ano de defesa: |
2023 |
| Autor(a) principal: |
Müller, Vanessa Ruviaro |
| Orientador(a): |
Rech , Virginia Cielo |
| Banca de defesa: |
Zago , Adriana Maria,
Martins , Mirkos Ortiz |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1255
|
Resumo: |
The present study focuses on assessing potential genotoxicity in the hepatic, renal, and splenic tissues of rats treated with a gel containing quercetin-loaded nanoparticles for muscle injury. Muscle injuries, commonly observed in the population, can result from direct traumas (such as physical contusigns) or indirect causes (associated with neurological dysfunctions), with physical activity being a potential risk factor. These injuries frequently exacerbate the production of reactive oxygen species, necessitating treatments to mitigate this process. New therapeutic approaches are being explored to alleviate damage arising from inflammatory pathways. Natural compounds have garnered significant interest in the scientific community, with quercetin, a polyphenol of the flavonoid class naturally found in foods like onions and apples, emerging as a promising candidate. However, its low bioavailability in therapeutic forms has prompted the utilization of nanoparticles to enhance absorption and aqueous solubility. Through agarose gel electrophoresis, we assessed double and single-strand DNA breaks in the tissues of animals subjected to treatments. Additionally, levels of reactive oxygen species in these tissues were evaluated using the dichlorofluorescein assay. Results indicate that treatment with quercetin-loaded nanoparticles did not induce DNA damage in double strands in the kidney and spleen, or in single strands in the liver, kidney, and spleen. Concerning the production of reactive oxygen species, nanoparticle treatment did not elevate their levels in the hepatic, renal, and splenic tissues compared to the control group. We conclude that the gel containing nanostructured lipid carriers with quercetin does not induce genotoxicity in renal and splenic tissues, but led to genotoxic effects in hepatic tissue by increasing double-strand breaks compared to the control. |
