Detalhes bibliográficos
| Ano de defesa: |
2023 |
| Autor(a) principal: |
Prade, Josiéle da Silva |
| Orientador(a): |
Cadoná, Francine Carla |
| Banca de defesa: |
Saccol, Ana Lúcia de Freitas,
Krause , Luciana Maria Fontanari |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Mestrado em Ciências da Saúde e da Vida
|
| Departamento: |
Ciências da Saúde e da Vida
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1221
|
Resumo: |
Type B ultraviolet radiation can favor the initial process of carcinogenesis. Therefore, natural products are being investigated to reveal new agents with photobiological action. Theobroma cacao L. known as cocoa, is a food rich in polyphenols and flavonoids with antioxidant, anti-inflammatory and anticarcinogenic effects. Therefore, the objective of this study was to investigate the in vitro photobiological action of cocoa solution on a fibroblast line (HFF-1) exposed to UVB radiation. Three experimental models were conducted where cells were treated with different concentrations of cocoa (50, 100, 250, 500, 750 and 1000 and 1500 µg/mL) and exposed to UVB (7 kJ/m2 for 10 minutes): 1st) analysis of the protective action of cocoa; 2nd) investigation of the protective activity of cocoa at the time of exposure; 3rd) study of the reversion activity of cocoa to the damage caused by UVB. After the incubation periods stipulated for each model, viability, oxidative stress and cell cycle analyzes were carried out. The results suggested that cocoa did not present significant activity in the blocking and protection model, but exhibited biological action in the reversal model at almost all concentrations tested when compared to cells exposed only to UVB. Furthermore, cells treated with cocoa alone, without exposure to UVB, showed higher levels of proliferation, suggesting that cocoa protects cells from basal oxidative stress and provides a more favorable environment for cell growth. This effect can be elucidated through the decrease in the levels of reactive oxygen species and nitric oxide, which were increased in cells exposed only to UVB. Furthermore, cocoa was able to modulate the cell cycle of cells without exposure to UVB, increasing the G2/M phase, although it did not generate significant changes in cells exposed to cocoa and UVB. Therefore, it can be concluded that cocoa improved basal cell proliferation rates and exhibited an important reparative activity against damage caused by UVB in human fibroblasts, and can be further explored in additional experiments with the aim of helping to repair damage caused by UVB. UVB and thus prevent diseases such as skin cancer. |
