Diversidade de Candida spp. isoladas de animais domésticos e silvestres e concentração inibitória mínima a antifúngicos

• Ano de defesa: 2017
• Autor(a) principal: Cândido, Stéfhano Luís
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Medicina Veterinária (FAVET); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Candidíase; Caracterização; Fungos; Isolamento; ITS; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Candidiasis; Characterization; Fungus; Isolation
• Link de acesso: http://ri.ufmt.br/handle/1/3358

Candida spp. are yeasts that are widely distributed and are considered to belong to the microbiota of the skin and mucous membranes of humans and animals. When there is imbalance of this microbiota or the immune system of the host is compromised, the species of this genus can develop, through its virulence factors, the candidiasis. The most common infections in animals are: urinary, peritonitis, keratoconjunctivitis and seborrheic dermatitis. The diversity of Candida spp. in wild and domestic animals is still little reported in Brazil. In view of this the objective of this study was to describe the diversity of isolates of Candida sp. In domestic and wild animals from materials received at the Laboratory of Veterinary Microbiology at the Federal University of Mato Grosso, campus Cuiabá and to verify the antifungal susceptibility profile of C. glabrata, C. tropicalis and C. parapsilosis isolates, associating the isolated species with their Isolation sites. The total of 79 isolates of Candida sp. Of 13 different injury sites of domestic and wild animals were studied. The isolates after previous characterization were submitted to DNA extraction, PCR amplification and sequencing of the ITS region. The sequences obtained were compared to the DNA database using the BLAST algorithm from the National Center for Biotechnology Information (NCBI). For the susceptibility test, 10 isolates of each species of C. glabrata, C. tropicalis and C. parapsilosis were submitted to the evaluation of the Minimum Inhibitory Concentration for Caspofungin, Fluconazole, Voriconazole, Amphotericin B, Nystatin and Itraconazole. The 79 isolates were identified in 12 different species. C. rugosa was the most frequent species in domestic animals with 39% (19/49), followed by C. parapsilosis with 26% (13/49); In wild animals C. albicans and C. glabrata were the most isolated both with 23% (7/30). In samples from the ear (26/79), C. rugosa was the most frequent species 14/26 (54%); In feces, was C. tropicalis 5/14 (35%). C. rugosa (4/7) and C. albicans (4/12) were more associated with milk and oral swab, respectively. Regarding the susceptibility profile, 100% of the tested isolates were resistant to Itraconazole and susceptible to Variconazole. It is concluded that the most frequent species of Candida was C. rugosa, followed by C. parapsilosis which indicates an increase in the isolations of non-albicans species. In addition the antifungal with greater susceptibility was the Variconazol and with lesser resistance was the Itraconazole that has been the most used in the Veterinary Medicine and in this way the treatments with this principle is ineffective.