Expressão da anexina-A1 em leucócitos de pacientes com hanseníase

• Ano de defesa: 2014
• Autor(a) principal: Ribeiro, Afonso Bezerra
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Medicina (FM); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências da Saúde
• Palavras-chave em Português: Hanseníase; Anexina-A1; Células polimorfonucleares; CNPQ::CIENCIAS DA SAUDE::MEDICINA; Leprosy; Annexin-A1; polymorphonuclear cells
• Link de acesso: http://ri.ufmt.br/handle/1/485

Leprosy is endemic, infectious disease of chronic evolution, granulomatous caused by M. leprae, which represents a serious public health problem in Brazil and in countries around the world. The aim of this study was to analyze the populations of neutrophils, monocytes, TCD4+ cells, TCD8+ and T regulatory peripheral blood of patients with leprosy in multibacillary clinical and paucibacillary forms as well as the expression of anti-inflammatory ANXA1 protein and the levels of IL -10. Quantification of leukocytes in peripheral blood of leprosy patients and controls (healthy individuals without clinical history of infection - negative control, and patient with tuberculosis - positive control) was performed by hemocytometer Neubauer chamber counting and differential blood smear. The determination of the phenotype of TCD4+, TCD8+ and Treg cells was performed by immunofluorescence. The expression of ANXA1 and IL-10 levels in plasma and leukocytes by way of immunofluorescence and ELISA. The results were analyzed by analysis of variance (One-way ANOVA) with Bonferroni post test. The associations between non-parametric data were analyzed by linear regression and the correlation coefficient of Spearman. The results showed that the total leukocytes in patients MB (22.93 ± 0.37 x 106 cells / ml) and BP (26.58 ± 1.12 x 106 cells / ml) showed a significant increase in cell number when compared the CS group (6.43 ± 0.74 x 106 cells / mL). The CP group showed a significant increase also in relation to the CP group (21.45 ± 0.38 x 106 cells / ml). The population of monocytes in MB patients was increased (1.45 ± 0.28 x 106 cells / mL) compared to the CS group (0.59 ± 0.70 x 106 cells / mL) and CP (0.70 x 106 ± 0.17 cells / mL). The group of PB (0.23 x 0.04 ± 106 cells / mL) compared to CS and CP group did not show a significant increase between the groups. Neutrophils of MB patients (14.04 ± 0.53 x 106 cells / mL) and the PB (14.28 ± 0.22 x 106 cells / mL) showed a significant increase from the CS (3.42 x 106 ± 0.36 cell / ml) and CP (1.21 ± 0.06 x 106 cells / ml). TCD4+ cells, an increase was observed in clinical forms MB (1.99 ± 0.29 x 106 cells / ml) and BP (1.89 ± 0.18 x 106 cells / ml) compared to healthy, positive controls. Regarding the TCD8+ cells, the number of leukocytes was more significant in MB (1.14 x 0.10 ± 106 cells / mL) than the CS group (0.62 ± 0.05 x 106 cells / mL ), while the PB group (0.28 ± 0.02 x 106 cells / mL) decreased compared to the CS group. In the population of Treg cells, the clinical form MB (1.85 x 106 ± 0.27) had a higher number compared to the CS group (0.56 ± 0.07 x 106 cells / mL) and CP (0.30 x106 ± 0.02 cells / mL). The CP group (0.29 ± 0.03 x 106 cells / mL) had not increased significantly when compared to the CS group and CP. The ANXA1 decreased in circulating leukocytes in patients PB and MB, but showed high levels released into the plasma, and the multibacillary patients had levels above the paucibacillary. Being a regulatory molecule of inflammation, its paracrine action might be enhancing the anti-migratory action and inhibiting pro-inflammatory action in circulating leukocytes in infection induced by M. leprae, particularly in MB patients. No correlation was observed between the expression of annexin-A1 and IL-10 levels in patients with leprosy, indicating that this protein is not involved in the production of this cytokine induction mechanism in circulating leukocytes.