Caracterização fenotípica e genotípica de Trichosporon spp. implicados em infecções em humanos: um patógeno emergente

Detalhes bibliográficos
Ano de defesa: 2004
Autor(a) principal: Raquel Maria Lima Lemes
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Brasil
ICB - DEPARTAMENTO DE MICROBIOLOGIA
Programa de Pós-Graduação em Microbiologia
UFMG
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/1843/37404
Resumo: Sixty-five samples of yeasts were analyzed, and 39 were confirmed as belonging to the genus Trichosporon, for the classic method and five excluded by genotyping, through the method of RAPD (Randomly Amplified Polymorphic DNA) for the oligonucleotides use, capable to differentiate the main species of the genus for the characterization of the area of ITS (Intergenic Space). The primer OPA2 made possible the identification of 4 species (T. asahii, T. asteroids, T. ovoides and T. mucoides). The profile of susceptibility of the analyzed samples was tested to six antifungic drugs: amphotericin B (Ampho B), 5-flucitosin (5-Fc), fluconazole (Flu), itraconazoel (Itra), terbinafine (Terb) and voriconazole (Vor), in agreement with the technique described by NCCLS (National Commitee for Control Laboratory Standard) and the method E-test. Trichosporon asahii and T. ovoides presented sensibility at 5-flucitosin, T. mucoides to Ampho B and to Vor, while T. asteroides demonstrated resistance the all of the tested drugs, except for Flu. MIC (minimum inhibitor concentration) for Terb varied from 0.031 – 8.0 mg/mL. All of the species presented resistance to Itra and sensibility to Flu (MIC90 48 hours). The sample of environmental origin and those isolated of superficial and systemic mycoses were sensitive to Ampho B, 5-Fc and Vor, while those originating from of cutaneous and subcutaneous mycoses presented resistance to the polienic drug. All of the samples presented resistance to Itra. Except for two of T. asahii (an isolated from subcutaneous infection and another of superficial), the others presented sensibility to Flu. The comparison among the results obtained by the method of NCCLS and E-test revealed larger discrepancies among the tests with 5-Fc and Itra. RAPD overcame the classic method in the identification of species of Trichosporon, necessary identification to aid to address to the treatment of the trichosporonosis, due to the variability of the susceptibility profile to the antifungals among the species, independent of the isolation site.