Desenvolvimento de método para análise de acroleína-DNPH em alimento, ar expirado e ar ambiente utilizando SPME-GC/MS
| Ano de defesa: | 2012 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Tese |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de Minas Gerais
UFMG |
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: | |
| Link de acesso: | http://hdl.handle.net/1843/SFSA-8YBTTT |
Resumo: | The carbonyl compounds (CC) are highly toxic compounds. Humanexposure to CC occurs from vapors inhalation, dermal absorption and through food ingestion. Therefore, knowledge of the concentration levels of carbonyl compounds is extremely important for the evaluation of environmental contamination and exposure nevertheless in most cases, it is limited to the concentration of formaldehyde and, sometimes, acetaldehyde, neglecting other pollutants no less harmful to human health. A growing concern has been focused to the population that spends most of the day indoors, for instance, houses, offices, shops and leisure environments. Exposure to these environments becomes more critical due to the longer exposure time and high concentrations of pollutants emitted from various sources. This study describes the developemt and validation of gas chromatography methods for determination of acrolein in samples of exhaled air, ambient air andfoods fried with different types of oil. For the analysis of exhaled air analytical curve was built with a standard gas generation system through a permeation method. The analyses were carried out by gas chromatography with mass spectrometry detector using the technique of solid phase microextraction (SPME) with polyacrilate fiber (PA). To analyze the breath acrolein has been used a method permeation a system for generating pattern gaseous acrolein to constructthe calibration curve. The samples were analyzed by gas chromatography with mass spectrometry detector using the technique of solid phase microextraction (SPME). Two derivatization methods with 2,4-DNPH were developed, one using derivation in solution, for food analyses and another using derivation on PA fiber the analyses of breath and ambient air. The developed methods were validated and applied to real samples of exhaled air from two groups of individuals, smokers and non-smokers. The results showed no statistically significant difference for the groups of smokers and nonsmokers. Average concentration of acrolein in exhaled air of non-smokers was lower when compared to that of smokers. In ambient air, the concentrations of acrolein were greater in parking lots due to emission of acrolein by burning fuels. The methodology developed allowed quantitative analyses of acrolein-DNPH; in various foods (potatoes, cassava, and sausage) fried in different types of oils.The values found in this study were smaller than the maximum established by the World Health Organization (WHO)-2002, which specifies that the concentration of acrolein in food does not exceed 40 g/g. The concentration of acrolein was lower at potatoes fried in soybean oil, due to the lowest content of unsaturated fatty acids present in this oil. In cassava and sausage the concentration of acrolein introducedusing corn oil were similar indicating that the oil absorption is independent of the food. The SPME method used for sampling and extraction of acrolein in different matrices showed appropriate, considering the simplicity and rapidity in the analytical process. Gas chromatography with mass spectrometry provided the selectivity, accuracy, sensitivity, and reliability required for the studies. All resultsfound for these parameters were appropriate for the purpose of study |