Expressão da Osteopontina após utilização de matriz dentináriahumana desmineralizada em alvéolos de ratos: análise imunohistoquímica

• Ano de defesa: 2016
• Autor(a) principal: Alexandre Moreira Fernandes
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Reparo; Matriz Dentinária Humana Desmineralizada; Alveolar; Reparo Ósseo; Osteopontina; Osteogênese; Dentina; Alvéolo dental
• Link de acesso: http://hdl.handle.net/1843/ODON-AJ4LDM

BACKGROUND - Despite its good capacity for regeneration, bone tissue subjected to some types of fractures or surgery that can lead to large bone defects. The major bone defects treatment strategies are based in osteoinduction or osteoconduction. Demineralized human dentin matrix (DHDM) is a biocompatible alternative to fill bone defect, improving the quantity and quality of bone produced.METHODS - Wistar rats were selected, submitted to the extraction of bothsecond molars (right and left). Alveoli were separated into two groups: control (right) filled with blood clot and experimental (left) filled with DHDM. Animals were sacrificed at 5, 10 and 21 days. Histological and histoquantitative analyzes (analysis of variance - ANOVA, and Tukey's test) were performed and immunostaining for osteopontin (OPN) as osteogenesis indicator. RESULTS - 5 days - DHDM incorporated by new trabeculae. 10 days - connective tissue organization and new trabeculae in the experimental group. Intense staining for OPN close to DHDM in the experimental group. 21 days - experimental group showing mature trabeculae. Statistical difference observed (p<0.05). Higher number of trabeculae in experimental groups in all periods of analysis.CONCLUSIONS - DHDM implanted in alveoli induces the acceleration ofosteogenesis. Presence of OPN observed more intensely at 10 days close to DHDM.