Estadiamento e caracterização da proteinúria em cães naturalmente infectados com Leishmania infantum

• Ano de defesa: 2021
• Autor(a) principal: Pillar Gomide do Valle
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA; Programa de Pós-Graduação em Ciência Animal; UFMG
• Palavras-chave em Português: Cão; Doença; Eletroforese; Leishmaniose visceral
• Link de acesso: http://hdl.handle.net/1843/36154; https://orcid.org/0000-0002-3736-6052

Canine visceral leishmaniasis (CVL), a disease with high prevalence in Brazil, has as one of its main consequences renal injury due to tubular and glomerular lesions resulting mainly from the deposition of immune complexes. The presence of asymptomatic infected animals hinders the diagnosis that must be made through the combination of physical, parasitological, serological and molecular tests. After diagnosis of the infection, its staging is essential for a better composition of the therapeutic management. For that, markers are needed that can indicate the evolution and prognosis of kidney disease, one of the most important occurrences in CVL, which causes death in many infected animals. Thus, in the search for an early biomarker of renal injury, this study aimed to determine the urinary protein profile of dogs infected with leishmaniasis treated and without treatment. For this, 30 dogs from an outpatient clinic at a Veterinary Hospital in Belo Horizonte were evaluated. All animals underwent clinical, ultrasound and laboratory tests that included renal biomarkers such as urinalysis, urinary protein and creatinine ratio (UPC), symmetrical dimethylated arginine (SDMA), blood urea nitrogen (BUN) and serum creatinine. In addition, urinary proteins were characterized using the SDS-PAGE electrophoresis technique, and thus, a urinary protein profile was developed comparing healthy, uninfected patients with dogs infected with Leishmania infantum with and without treatment. The results showed that the hematological and biochemical parameters showed similar behavior between the groups of uninfected dogs and treated infected dogs, however a very heterogeneous pattern of urinary proteins can be observed and differed between healthy non-infected animals and animals infected with L. infantum with or without treatment. The results suggest that the classification of proteinuria can be a tool that helps in the staging of animals infected with L. infantum and can differentiate them in terms of the severity of existing kidney injuries