Leishmaniose Visceral Canina: Doença de Caráter Fibrosante

• Ano de defesa: 2010
• Autor(a) principal: Lucelia Coimbra da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Patologia; Linfonodos/patologia; Rim/patologia; Baço/patologia; Matriz extracelular; Pulmão/patologia; Leishmaniose visceral/imunologia; Imunoistoquímica; Leishmania infantum/patogenicidade; Fígado/patologia; Leishmaniose visceral/patologia; Cães
• Link de acesso: http://hdl.handle.net/1843/BUOS-8HEPRU

The aim of this work was study the extracellular matrix alterations in liver, spleen, cervical lymph nodes, lungs and kidneys in symptomatic dogs naturally infected with Leishmania (Leishmania) chagasi correlating with clinical and anatomical pathological aspects. This study was carried out with 30 dogs, divided at two groups: six not infected animals (group control) and twenty four symptomatic infected animals. All them was mongrel dogs with undefined age, obtained from the municipality of Santa Luzia/MG and Ribeirão das Neves/MG municipalities. The group denominated symptomatic was composed by animals with classical clinical signals of the disease as skin lesions (alopecia, eczemas and ulcers), loss weight and lymphopathy. Paraffined sections of the tissues were stained by Hematoxylin-Eosin (HE); Gomori's ammoniacal silver staining for reticular fibers and strepto-avidin peroxidase Immunohistochemical method for tissue Leishmania amastigotes detection. The tissue images were transferred to a computer video screen by means of the software KS300 and relayed to a computer-assisted image analysis system (Kontron Elektronic/Carl Zeiss, Germany) for morphometrical analysis. A significant increasing of collagen deposition in all organs was found when compared to the controls. Positive correlation between the parasite load and collagen deposition was found in all organs expect the lungs of infected animals. The organs that showed higher colagenogenesis were livers, kidneys and lungs. Our results have indicated that canine visceral Leishmaniasis is a fibrotic disease model.