Detalhes bibliográficos
| Ano de defesa: |
2014 |
| Autor(a) principal: |
Rodrigues, Amanda Rafaela
 |
| Orientador(a): |
Ulhoa, Cirano José
|
| Banca de defesa: |
Ulhoa, Cirano José,
Monteiro, Valdirene Neves,
Fernandes, Kátia Flavia |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Biologia (ICB)
|
| Departamento: |
Instituto de Ciências Biológicas - ICB (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/4912
|
Resumo: |
The genus Trichoderma is represented by non-pathogenic soil fungi that have been studied by act as biological control agents against fungal pathogens, such as Sclerotinia sclerotiorum, the fungus that causes white mold, besides that, reduce environment and human health risks by being able to replace agrochemicals. Proteomic strategies with MS techniques have been important tools in studies of patterns identification for protein expression in different growth conditions. This work aims to development new strategies that enable the detection and identification of proteins secreted by Trichoderma asperellum (TR356) and Sclerotinia sclerotiorum when inoculated together and separated. The results obtained by MALDI / TOF analysis allowed the identification of a β-1.3--glucanosiltransferase and α-1.2-Dmannosidase, demonstrating the possibility of proteins identification for better comprehension about interaction between these organisms. It was therefore possible to identify the proteins through strategies used, but further analysis are required in order to elucidate the function and interaction of proteins secreted by the fungus Trichoderma asperellum TR356 against S. sclerotiorum. |
