Efeito da fonte de carbono e nitrogênio na produção de β 1,3 glucanases por Trichoderma asperellum

• Ano de defesa: 2008
• Autor(a) principal: SILVA, Regiane Christine da
• Orientador(a): ULHOA, Cirano Jose
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Biologia
• Departamento: Ciências Biolóicas
• País: BR
• Palavras-chave em Português: Trichoderma asperellum; b-1,3-glucanases; fonte de carbono e nitrogênio; 1.Fungo(Trichoderma asperellum) - Controle biológico; 2.Enzimas hidrollíticas;
• Palavras-chave em Inglês: β -1.3 - glucanases; carbon and nitrogen source; Trichoderma asperellum.
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1279

The β-1.3-glucanases have several functions in the cell as fungal metabolic in hydrolysis of polysaccharides for possible cellular assimilation; morphogenetic function in the hydrolysis or modifications of the cell wall, which includes growth and extension of the wall, altering the structure and composition of the wall and autolysis. In addition to presenting ecological importance, since these enzymes are involved in biological control, through the process of mycoparasitism. The gender Trichoderma comprises a group of filamentous fungi, saprophytic soil, found on decomposing organic matter in the rhizosphere, and some plants. Considering the importance of β-1.3 glucanases proposing in this study to evaluate the production of β-1.3 - glucanases by T. asperellum using different sources of carbon and nitrogen. The enzyme was produced using cell wall of Rizoctonia solani (PCRS), chitin, chitosan, starch, cellulose, sucrose, maltose, lactose, celobiose and glucose. The best production of enzymes was obtained in the middle containing PCRS, with two bands of activity of polyacrylamide gel were found. Furthermore, the effect of varying concentrations of ammonium sulfate (2, 4, 6, 8, 20, 40 and 60 mM) in the production of β-1.3-glucanase was evaluated during the growth of the fungus in PCRS. A great increase in activity of the enzyme was detected in the presence of nitrogen source. We can see that there was a considerable increase in the expression of the enzyme of low molecular weight at this culture conditions.