Detalhes bibliográficos
| Ano de defesa: |
2023 |
| Autor(a) principal: |
Nascimento, Tiago Lemos do
 |
| Orientador(a): |
Amaral, André Corrêa
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| Banca de defesa: |
Amaral, André Corrêa,
Silva, Daniela de Melo e,
Curcio, Juliana Santana de |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
|
| Departamento: |
Instituto de Ciências Biológicas - ICB (RMG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/13349
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Resumo: |
Fungi of the genus Paracoccidioides are the causative agents of Paracoccidioidomycosis (PCM), an infection prevalent in Latin America that mainly affects workers whose work activity is land management. Paracoccidioides spp. have their cell walls during the yeast phase, consisting mainly of chitin, a polymer formed by β-1,4- glycosidic bonds. Humans are capable of producing chitotriosidase (CHIT-1), an enzyme that has the ability to hydrolyze these bonds present in chitin. CHIT-1 is an enzyme encoded by the CHIT1 gene, with an important role in immune defense against chitin-containing pathogens, such as fungi. Polymorphism containing the duplication of 24 base pairs in exon 10 of chromosome 1 has been associated with decreased CHIT-1 production. Therefore, this study aimed to evaluate the prevalence of the 24 bp duplication polymorphism in CHIT1 in 138 individuals, divided into four groups. Group I: patients treated at the State Hospital for Tropical Diseases – Dr. Anuar Auad (HDT) in Goiânia with a confirmed diagnosis of PCM, Group II: researchers who during their research manipulated the fungus and without a confirmed diagnosis of PCM. Group III: rural workers without a confirmed diagnosis of PCM. Group IV: people without a confirmed diagnosis of PCM. The identification of the gene polymorphism was carried out using the polymerase chain reaction (PCR) technique, observing the size of the amplicons in agarose gel. The prevalence of the 24 bp duplication in exon 10 of the CHIT1 gene in the total population was 55.1% for the homozygous wild genotype, 40.6% for the heterozygous and 4.3% for the homozygous mutant genotype |
