Imunofenotipagem e avaliação quantitativa de linfócitos circulantes de bovinos da raça curraleiro

• Ano de defesa: 2008
• Autor(a) principal: MORAES, Júlia de Miranda
• Orientador(a): BRITO, Luiz Augusto Batista
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Ciência Animal
• Departamento: Ciências Agrárias - Veterinaria
• País: BR
• Palavras-chave em Português: imunocitoquímica, CD3, LB, leucócitos, pé-duro, perfil imunológico, leucograma; Bovino Raça [curraleiro]- Resistência a doenças; Bovino Perfil imunológico;. Bovino - Imunocitoquímica; Bovino Leucócito
• Palavras-chave em Inglês: immunocytochemistry, CD3, LB, leukocytes, immunological profile,leucogram.
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/947

The Curraleiro cattle are extremely docile and resistant to infectious diseases and parasites. They may be used in exploration of low-quality pastures, without great investments, when other breeds could show a low productivity or even survival difficulties. This genetic potential runs risk to extinction due to its replace by more productive breeds. The Curraleiro cattle resistence to illness is popularly known and many works has been published about it, although its immunologic system physiology is completely unknown. Such features are plausible reasons for the preservation of this potentially genetic resource. So, the mean aim of this study was to establish an immunological profile by marking and quantification of T and B lymphocytes in Curraleiro breed with immunocytochemistry. Thus, it was used 116 bovines, male and female, with different ages from two farms situated at Goiás State, Brazil. The animals were allotted in groups according to age, sex and origin. Blood samples were collected and processed in accordance with immunocytochemistry standard technique using lymphoid markers species-specific, anti-CD3 (MM1A BoCD3) and anti-LB (LCTB16A clone B-B14), for T and B lymphocytes counting, respectively. All procedures were accomplished at Veterinary School, Federal University of Goiás, Brazil. The data were submitted to descriptive statistics and then to Kruskall Wallis and Mann-Whitney tests. The results showed decreased levels of leukocytes, lymphocytes, T lymphocytes and B lymphocytes along the age advance. Absolute values of leukocytes, lymphocytes and T lymphocytes were higher in males than females. None of the evaluated parameters were affected by differences of the management carried out at two farms.