Análise imuno-histoquímica do colágeno tipo I em carcinoma de células escamosas orais e suas lesões precursoras

• Ano de defesa: 2017
• Autor(a) principal: Fanchiotti, Renata Escapini
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Clinica Odontológica; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Clínica Odontológica
• Palavras-chave em Português: Squamous cell carcinoma; Extracellular matrix; Type I collagen; Matriz extracelular; Colágeno tipo I; Carcinoma de células escamosas; Colágeno; Odontologia; 616.314
• Link de acesso: http://repositorio.ufes.br/handle/10/8119

Squamous cell carcinoma (SCC) is the most common cancer of the mouth, and is still associated with high mortality rates. Besides the evaluation of the clinical aspects, it is fundamental to perform a more accurate analysis of the molecular profile to better understand its biological behavior. Type I collagen is one of the main components of the extracellular matrix (ECM) and has been associated with the process of tumorigenesis by participating in events such as angiogenesis and metastasis. The present study performed an immunohistochemical analysis of the type I collagen profile in lesions diagnosed by the Department of Oral Pathology of the Dental School / UFES. The groups evaluated were: 10 cases of low risk lesions; 10 cases of high risk of malignancy and 30 cases of SCC. Also, associations were established between the markers and the clinical profile of the patient and the tumor. Our findings showed that men were the most affected in the lesions at high risk (60%) and SCC (86.6%), while women represented the majority in low risk lesions (80%). In relation to the affected sites, the jugal mucosa was the most frequent in the low risk lesions (50%), and the tongue in lesions of high risk (60%) and SCC (43.3%). As for the profile of type I collagen, the fibers were moderately irregular (C ++) and strongly marked. In the low risk lesions, the three types of fiber disposition were observed, and with weak marking; And those at high risk, the fibers were fine, regular and markedly strong. When the association between clinical and microscopic findings was found, it was possible to observe that patients smokers with SCC, the fiber pattern was irregular (P = 0.04) and strong marking (P = 0.02) compared to smokers, but diagnosed with lesions Low or high risk. Our findings suggest associations between clinical and microscopic aspects regarding the pattern of deposition of type I collagen during the alterations that occur in the oral mucosa in the process of malignant transformation.