Avaliação físico-química de nanopartículas de ouro reduzidas com citrato de sódio e capeadas com Virola oleifera

• Ano de defesa: 2017
• Autor(a) principal: Corrêa, Andressa dos Santos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Bioquímica e Farmacologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Bioquímica e Farmacologia
• Palavras-chave em Português: Metallic nanoparticles; Antioxidants; Immunologic citotoxicity; Nanopartículas metálicas; Antioxidantes; Citotoxicidade imunológica; Farmacologia Bioquímica e Molecular; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/7869

The capping of gold nanocrystals using sodium citrate with Virola oleifera was the focus of this thesis, which aim/aiming at synthesize and characterize evaluate the final extract concentration and pH influence in surface particle adsorption. Furthermore, was analysed antioxidant activity and citotoxicity of AuNPs, for investigate your potential biological application. For this goal, the best capping time, and subsequently the optimum pH range and final extract concentration was determined and was realizes a 32 factorial design with 3 levels and 2 variables (pH and extract concentration) to verify the optimal capping conditions. In this way, it has been proved the adsorption was most intense between pH 3 and 5 and the most stable was the lower final extract concentration. The x-ray diffraction and the transmission electron microscopy confirmed the face-centered cubic structure of citrate AuNPs and the quasi spherical aspect, respectively. The adsorption of aromatic molecules was confirmed by spectroscopy techniques: Fourier-Transform Infrared (FTIR) and Raman. The DPPH and FRAP analyzes shows enhancement of antioxidant activity with capping of citrate gold nanoparticles. Furthermore, the citotoxicity assay shows low citotoxicity of capped gold nanoparticles.