Existe concordância entre o nested PCR e o 4Dx® Plus no diagnóstico de Erliquiose canina?

• Ano de defesa: 2019
• Autor(a) principal: Oliveira, Brenda Fayla Seco de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Ciências Veterinárias; Centro de Ciências Agrárias e Engenharias; UFES; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Ehrlichia canis; Erliquiose monocítica canina; ELISA; Sensibilidade; Biologia molecular; Teste imunoenzimático; Ciências Agrárias
• Link de acesso: http://repositorio.ufes.br/handle/10/11192

Canine monocytic ehrlichiosis is a hemoparasitosis caused by the intracellular bacterium Ehrlichia canis, which has a worldwide distribution, although it occurs more frequently in regions with tropical and temperate climates due to the high prevalence of its biological vector, the Rhipicephalus sanguineus. Due to the high prevalence of ehrlichiosis in dogs, the severity of this disease and the need for a more precise diagnosis, the aim of this study was to evaluate the diagnostic value of the serological test (4Dx® Plus) in relation to the nested PCR and to correlate the hematological findings with the nested PCR in the diagnosis of ehrlichiosis. Eighty-three suspected dogs were selected, without history of previous hemoparasitosis. Hemogram, blood smear (detection of Ehrlichia morulae), ELISA test (4Dx® Plus) and nested PCR were performed. Among 83 samples, nested PCR was positive for Ehrlichia canis in 26 dogs (31.33%). Evaluation by the 4Dx® Plus test showed that 39 dogs were positive (46.99%) for Ehrlichia sp. It was observed that only 15 dogs (18.07%) presented positive results, both in nested PCR and 4Dx® Plus test, presenting a poor agreement. It was observed that 4Dx® Plus test presented a sensitivity of 57.69%, specificity of 57.89%, with an accuracy of 57.83%. There was no correlation between the hematological parameters with the presence of Ehrlichia canis diagnosed in the nested PCR. It is concluded that there is a great disagreement between the results obtained in the nested PCR and the 4Dx® Plus and, considering the nested PCR as the gold standard for the diagnosis of ehrlichiosis, a low accuracy, sensitivity and specificity of 4Dx® Plus.