Caracterização físico-química de hidrolases de proteoforma de tripsina bovina e lipases de resíduos pesqueiros

• Ano de defesa: 2023
• Autor(a) principal: Cruz, Fabiano Torres
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Doutorado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Tripsina; Lipase; Caracterização enzimática; Biotecnologia
• Link de acesso: http://repositorio.ufes.br/handle/10/12469

Hydrolases have significant biotechnological importance, with lipases and proteases being highlighted as essential catalysts in industrial biotechnological processes, playing fundamental roles in the production of food, beverages, biofuels, and pharmaceuticals, with positive impacts both economically and environmentally. Integrating two chapters dedicated to the detailed study of trypsin and lipase, the research aims to deepen the understanding of their properties and contributions to the evolution of biotechnology. Notable advances in enzyme research have revealed a new proteoform of bovine trypsin, showing significant differences in activity, kinetic properties, and conformational stability compared to other known forms, opening new perspectives for understanding physiological pathways of enzyme inactivation. In the context of lipases, the study focuses on the economic and environmental potential of lipases derived from tilapia viscera, exploring purification, enzyme shelf life, and economic and environmental improvements. The results indicate that lipases from tilapia viscera can be purified and become viable alternatives with economic and environmental potential for Brazil.