Avaliação mecânica de Fibrina Rica em Plaquetas (PRF) de pacientes sob uso de varfarina

• Ano de defesa: 2022
• Autor(a) principal: Marinho, Erasmo Bernardo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Fibrina Rica em Plaquetas; Varfarina; Anticoagulantes; Materiais Biocompatíveis; Testes Mecânicos
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/63591

The use of platelet-rich fibrin (PRF) has been widespread in dental practice in recent years. The present laboratory study aimed to evaluate mechanically PRF in patients using warfarin. Twenty-one subjects using warfarin (mean INR 2.30±0.89) and 21 non-anticoagulated subjects (mean INR 1.08±0.07) participated in the study, which gave rise to the PRF samples. Three test groups were formed, adopting blood centrifugation for 10, 14 or 18 minutes: groups A (n=21), B (n=21) and C (n=20), respectively. A control group consisted of non-anticoagulated PRFs, centrifuged for 10 minutes (n=21). Each PRF had its mass measured. Mechanical properties were evaluated by axial tensile test and suture strength test using the INSTRON 3345® universal testing machine. Hematological parameters were compared by the Mann-Whitney test, and mechanical parameters by the Kruskal-Wallis test followed by Dunn's post-test. Correlation was analyzed using the Spearman test (confidence of 95%). Groups A (2.17±0.86g) and B (2.21±0.66g) had greater mass than the control group (1.92±0.56g) with statistical significance. All groups had statistically similar axial tensile test results. Lower values of deformation in maximum force of the suture strength test were observed with statistical difference in groups A (107.07±25.05%) and C (104.81±16.79%) compared to the control (118.01±17.61%). Lower values of maximum force (0.12±0.05N), deformation at maximum force (97.59±19.08%) and force at break (0.08±0.05N) were observed in the suture strength test with statistical difference in group B compared to control (0.20±0.06N; 118.01±17.61%; 0.14±0.06N, respectively). PRF formation was possible even in patients on anticoagulant therapy. No significant impact was observed on the values of the axial tensile test in the three test groups. There was a negative impact of anticoagulation in the use of suture. In the present study, PRF from patients using warfarin was shown to be a biomaterial with a viscoelastic and moldable behavior, with a high capacity for deformation. Axial tensile test demonstrated PRF behavior of patients using warfarin similar to that of non-anticoagulated patients at all centrifugation times. However, suture strength test showed a difference in the PRF behavior of anticoagulated individuals, at all centrifugation times, compared to non-anticoagulated individuals.