Influência da fibrina rica em plaquetas em procedimento endodôntico regenerativo em molares de ratos

• Ano de defesa: 2023
• Autor(a) principal: Marina Verçosa Assumpção Soares da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA; Programa de Pós-Graduação em Odontologia; UFMG
• Palavras-chave em Português: Fibrina rica em plaquetas; Terapia regenerativa; Osteocalcina; PCNA; Proloterapia; Antígeno nuclear de célula em proliferação; Endodontia
• Link de acesso: http://hdl.handle.net/1843/65178

This study evaluated the influence of injectable platelet-rich fibrin (i-PRF) on repair after regenerative endodontic procedure (REP) in immature molars of rats. Right or left upper molars of 18 rats were divided into groups (n = 6): IB – the pulp tissue of the mesial canal was removed, irrigation with sodium hypochlorite and ethylenediaminetetraacetic acid was performed, and intracanal bleeding (IB) was induced; i-PRF – after the irrigation protocol, i-PRF was inserted inside the root canal, without inducing of IB; i-PRF-IB – the treatment was carried out as in the i-PRF group and then the IB was induced. At the end, the teeth were sealed. To produce the i-PRF, another 3 animals were used for intracardiac blood collection, and the blood was centrifuged (1200 rpm, 8 min). After 21 days, the animals were euthanized and the samples were prepared for histological and immunohistochemical analysis for proliferating cell nuclear antigen (PCNA) and osteocalcin (OCN). Statistical test was applied (P < 0.05). There was formation of mineralized tissue in root length or thickness in all groups (P > 0.05). The formation of connective tissue in the canals occurred up to the medium third in most IB specimens, and up to the cervical third in i-PRF and iPRF-IB (P < 0.05). There were odontoblast-like cells in the apical third of half of the PRF specimens, and apical and medium thirds in most of the i-PRF specimens; these cells were not found in the IB group (P < 0.05). The i-PRF and i-PRF-IB group had significant higher number of PCNA-positive cells (P < 0.05), and higher OCN immunolabeling, mainly i-PRF-IB compared to IB group (P < 0.05). It is concluded that i-PRF helped the repair process after REP in rats, inducing connective tissue formation in root canals, presence of odontoblast-like and PCNA-positive cells, and OCN immunolabeling, mainly when associated with IB.