Efeitos da copaifera langsdorffii desf. na isquemia- reperfusão de retalhos cutâneos randomizados em ratos

• Ano de defesa: 2007
• Autor(a) principal: Silva, Joaquim José de Lima
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Antioxidantes; Estresse Oxidativo; Retalhos Cirúrgicos
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/13585

The protective effect of Copaifera langsdorfii (Desf.) resin-oil was evaluated on an experimental model of random skin flap on rat’s dorsum. Seventy-two young Wistar rats, average weight 216,8g, were randomized in 4 equal (n=18) groups (GCS – Saline control, GCV – vehicle control, GT200 - Test 1 and GT400 – Test 2). Each group was redistributed in 3 subgroups, n=6 animals). All surgical procedures were performed under general anesthesia (ketamine 90 mg+Xylasine 10mg/kg, i.p.) Resin-oil and control drugs (saline and Tween 80) were administered by gavage 24, 12 and 2 hours prior to the beginning of the experiment followed by daily doses for the next 7 days. Tissue and blood samples were collected at the end of the surgical procedure (T-0) and 24 (T24)/168 (T168) hours later. Laboratory analyses included plasma and tissue levels of tiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) and tissue levels of myeloperoxidase (MPO). The Kolmorogov-Smirnov test was used for verifying samples homogeneity and comparisons were made using ANOVA/Tukey and Kruskal-Wallis/Dunn tests. P values <0.05 were considered significant. Tissue TBARS levels were significantly increased in T24 time point in rats treated with 200/400mg of resin-oil. Tissue TBARS levels increased and GSH levels decreased significantly in GCS compared with GCV during both ischemia and reperfusion. These findings suggests a possible pro-peroxidative and pro-oxidant effect of the vehicle (Tween 80). Plasma GSH levels increased significantly in T200 and T400 resin-oil treated rats and only in T400 rats tissues. These results point to a dose-dependent anti-oxidative effect of the resin-oil in tissues. MPO levels increased in GCV compared with saline control. This may represent a pro-inflammatory activity of the vehicle used for resin-oil dilution. Anti-inflammatory properties of copaíba oil were demonstrated as MPO concentrations decreased significantly in resin-oil treated rats compared with GCS and GCV. It is concluded that copaiba resin-oil exerts a discrete anti-lipoperoxidative activity and an intense anti-oxidative activity during ischemia/reperfusion of rat’s dorsum random skin flap