Desenvolvimento de nanocápsulas poliméricas contendo óleo essencial de alecrim-pimenta (Lippia sidoides Cham): caracterização e avaliação biológica

• Ano de defesa: 2016
• Autor(a) principal: Louchard, Bianca Oliveira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Lippia; Anti-Infecciosos; Óleos Voláteis; Nanopartículas
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/50378

The species Lippia sidoides Cham., known as rosemary-peppermint, is an aromatic plant of northeastern Brazil whose research show promising antimicrobial activity and anti-inflammatory. From the leaves of L. sidoides extract an essential oil consisting mainly thymol, pronounced antimicrobial component, but it can present toxicity at high doses. The placement of drugs in nanoparticulate form have attracted great interest in recent years because these systems are able to confer protection active substance, increasing the therapeutic efficacy and reducing side effects. The aim of this study was to develop, characterize and perform nanosystems biological assessment containing essential oil of L. sidoides (OELS). The study included characterization of plant and drug derivative (essential oil), analytical standardization - HPLC-DAD and GC-MS (marker: thymol), development of nanocapsules L. sidoides (NCLS), stability studies, and evaluation of cytotoxicity and the antimicrobial, antioxidant and anti-inflammatory topical. The OELS content extracted from the leaves was around 4.6% (major compounds: thymol 65%, o-cymene 17% and ecaryophyllene 4%). The analytical method by HPLC-DAD showed specific, linear (r = 0.996), accurate, precise and robust according to ANVISA guidelines. The NCLS (thymol: 3.1 ± 2.5 mg / mL) showed 92% encapsulation efficiency, average diameter 190nm, 0.204 PDI and zeta potential -20 mV. In the stability study there was no significant change of the parameters up to 120 days (25°C). In antimicrobial evaluation OELS and NCLS showed MIC 70 mg / mL. Assessment of antiinflammatory activity demonstrated that OELS and thymol depending on the dose has pro-inflammatory or anti activity. The nanosystems no active showed antiedematogenic effect, and among the investigated nanosystems, NCLS and nanoemulsion / OELS, the latter significantly inhibited the ear edema induced by TPA and reduced the concentration of myeloperoxidase (MPO). The development and standardization of nanosystems the basis of L. sidoides showed technological features of interest, quenched toxicity OELS in maintaining human neutrophil antimicrobial potential antioxidant and anti-inflammatory plant.