Análise in silico da dependência metabólica entre Angiostrongylus spp. e seus hospedeiros

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Ramos, Amaranta Rangel
Orientador(a): Graeff-Teixeira, Carlos
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul
Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Celular e Molecular
Departamento: Escola de Ciências Saúde e da Vida
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://tede2.pucrs.br/tede2/handle/tede/10386
Resumo: Two species of Angiostrongylus, A. cantonensis and A. costaricensis, are known to cause eosinophilic meningitis, due to the presence of the parasite in the larval stage in the brain, and abdominal angiostrongyliasis, due to the presence of adult worms in the mesenteric artery, in humans that were accidentally infected. The capability of the parasites to remain within the host may be related to the ability to obtain nutrients and to utilize molecules fundamental for the development of the parasite, however, such responses may be different according to host genus. With the premise that the parasite uses molecules synthesized by its host to complement its own metabolism, the present study aims to evaluate through the annotation of metabolic pathways using AnEnPi and KAAS programs the metabolic dependence of two species of Angiostrongylus spp. in their hosts. When analyzing the proteomes, nine enzymes were identified only in A. cantonensis and one in A. costaricensis, as well the presence of 13 enzymes with the same function in the parasites and their hosts. We found 156 enzymes that showed a possible metabolic dependence between A. cantonensis and A. costaricensis and their hosts Rattus norvegicus, Mus musculus, Biomphalaria glabrata and Homo sapiens. Among the enzymes that possibly represent metabolic dependence were found seven enzymes with characteristic lethality, and one enzyme that also was not present in all the parasite organism analyzed.