Marcadores protéicos do carcinoma epidermóide de cabeça e pescoço com fenótipo invasivo

• Ano de defesa: 2009
• Autor(a) principal: Vidotto, Alessandra
• Orientador(a): Silva, Eloiza Helena Tajara da
• Banca de defesa: Souza, Dorotéia Rossi Silva , Nogueira, Maurício Lacerda , Greene, Lewis Joel , Gozzo, Fabio Cesar
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Faculdade de Medicina de São José do Rio Preto
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências da Saúde
• Departamento: Medicina Interna; Medicina e Ciências Correlatas
• País: BR
• Palavras-chave em Português: Neoplasias de Cabeça e Pescoço; Metástase Neoplásica; Linfonodos; Saliva; Soro; Proteômica; Eletroforese em Gel Bidimensional; Espectrometria de Massas; Marcadores Biológicos
• Palavras-chave em Inglês: Head and Neck Neoplasm; Metastasis, Lymph Node; Saliva; Serum; Proteomics; Two-dimensional Electrophoresis; Mass Spectrometry; Biological Markers; Neoplasm Metastasis; Lymph Nodes
• Palavras-chave em Espanhol: Neoplasias de Cabeza y Cuello; Metástasis de la Neoplasia; Ganglios Linfáticos
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::CIRURGIA::CIRURGIA OTORRINOLARINGOLOGICA
• Link de acesso: http://bdtd.famerp.br/handle/tede/125

The regional lymph nodes play a pivotal role in diagnosis, staging and management of head and neck squamous cell carcinomas (HNSCC). Despite their importance, detailed understanding of the probable mechanisms of lymphatic metastases has not been completely achieved. Subjects and Methods: We analyzed metastatic and normal lymph node tissues, as well as saliva and serum from sixth-two patients with HNSCC, and twenty-nine controls using two-dimensional electrophoresis, MALDI-Q-TOF and western blot. Results: Several proteins were found to be significantly increased in metastatic nodes, such as stratifin, glutathione S-transferase pi, apoliproteín A-I, alpha-1-microglobulin, disulfide isomerase, galectin, citokeratins, immunoglobulins, transtirretin, calciun-binding protein (família S100) and fat-binding protein (FABP). Among the down-regulated proteins in metastatic lymph nodes are calreticulin, tropomiosin 3, triosephosphate isomerase, piruvate quinase, anidrase carbonic, gamma actin, peroxiredoxin 2, profilin 1, gliceraldeyde 3- fosfato desidrogenase and heat shock proteins. These proteins are involved in epidermis development, cell proliferation, migration and adhesion, apoptosis, defense and inflammatory response and xenobiotic metabolism. Our data on the expression of heat shock proteins and enzymes of the glycolytic pathway suggest an effect of the lymph node environment in controlling tumor progression or in metabolic reprogramming of the metastatic cell. In saliva, 13 proteins showed an altered pattern of expression in samples patient, including over-expression of keratins, immunoglobulins, alphaamylase, PLUNC and zinc-alpha-2-glycoprotein and down-regulation of myosin. In serum samples, six proteins were over-expressed (serum albumin, alpha-1- microglobulin/bikunin precursor, apolipoprotein A-I, haptoglobin, serotransferrin, transthyretin) and two were under-expressed (hemoglobin subunit alpha, hemoglobin subunit beta) compared to the control group. Conclusion: New potential markers, such as profilin-1 and E-FABP, were identified and may be proved useful for defining the invasive phenotype of head and neck carcinomas.