Estudo da relação entre homeostase de ferro, estresse oxidativo e inflamação: do modelo molecular ao treinamento de força periodizado e não periodizado

• Ano de defesa: 2019
• Autor(a) principal: Sampaio, Ricardo Camões
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Cruzeiro do Sul; Brasil; Campus Liberdade; Doutorado Interdisciplinar em Ciências da Saúde; Cruzeiro do Sul
• Palavras-chave em Português: Homeostase de ferro; Estresse oxidativo; Citocinas; CNPQ::CIENCIAS DA SAUDE
• Link de acesso: http://repositorio.cruzeirodosul.edu.br/handle/123456789/319

Iron is the most abundant essential metal in living organisms and it has a fundamental physiological and metabolic roles in animals and humans. On the other hand, iron might be harmful when its homeostasis is disturbed, as iron ions are main catalysts for the formation of Reactive Oxygen and Nitrogen Species (RONS). The aim of this study was to investigate the relationship between iron homeostasis, physiological markers, and inflammatory cytokines in strength training subjects (ST). The study also addressed molecular in vitro aspects, by simulating resting or exhaustion conditions in plasma (or interstitial) after ST practice, focusing on the catalysis of heme proteins, hemoglobin and myoglobin, in the oxidative insult. Twenty-six male subjects, between 20 to 45 years-old, were divided in 3 training groups: linear periodized training (PL), non-linear periodization (PNL) and non-periodized (NP), for 10 consecutive weeks. Results in vitro suggest that increasing concentrations of lipidic peroxides induce proportional increases of peroxidase activities of myoglobin even at rest conditions, which may culminate in increases of oxidative stress. In humans, although observed increases in iron concentrations after the tests at 80% of a maximal repetition, they were less expressive during weeks 3, 12 and 13, respectively (NP= 92,32%, 87,10% e 71,88%; PL =65,01%, 38,31% e 23,86%; PNL= 86,35%, 79,21% e 76%). The iron-binding capacity of PL subjects was significantly increased in the post test of week 12. The concentrations of TNF-α showed significant increases in post tests for NP (week 3) We can suggest that the ST protocols applied here promoted iron homeostasis rebalances with proportional pro-inflammatory cytokine responses at model NP upon strength test performed along the study.