Aspectos sazonais da fisiologia da reprodução do macho de lobo-guará (Chrysocyon brachyurus, Illiger 1811) de cativeiro

• Ano de defesa: 2011
• Autor(a) principal: Oliveira, Laura Teodoro de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Mestrado em Cirurgia e Anestesiologia Veterinária; UNIFRAN
• Palavras-chave em Português: Colheita de sêmen; Manipulação digital; Sazonalidade; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/687

The present study aimed to expand knowledge about the reproductive physiology of the male maned wolf (Chrysocyon brachyurus, Illiger 1811) in captivity, through the study of the reproductive cycle and evaluation of hormonal concentrations, collection and semen characterization. Three adult males, with no reproductive history, were studied for a period of 13 months, at the Scientific Breeding Ground of the Brazilian Company of Metallurgy and Mining, in Araxá, MG. Semen collections were performed by means of digital manipulation of the penis and 100% success was achieved in the reproductive season (ER) and 83.78% in the non-reproductive season (ENR). Of the ejaculates collected, 78.33% contained cells in the ER, while 38.70% of the ENR contained them. The ejaculate showed only a fraction, without odor, of translucent color (72.40%), whitish (10.75%) and white (8.60%), with all samples having a watery appearance, with a greater number of samples. translucent and whitish staining in the ER and translucent in the ENR (p = 0.039). The pH was 6.7 in both ER and ENR. There was a significant difference (p <0.05) in the mean values ​​of the volume of the ejaculate (1.34ml in the ER and 0.81ml in the ENR), total sperm concentration (57.74x106 in the ER and 13.19x106 in the ENR) , concentration of mobile sperm (63.58x106 in the ER and 21.85x106 in the ENR) and percentage of live (82.13% in the ER and 72.57% in the ENR) between seasons. A high percentage of major defects was observed in the two seasons, with a higher frequency of defects with a strongly folded or curled tail (12.39% in the RE and 19.17% in the ENR), underdeveloped forms (9.36% in the RE and 5, 92% in ENR), intermediate part defects - such as fracture and thickening (9.22% in RE and 11.83% in ENR), abnormal small head (5.87% in RE and 8.00% in ENR) and proximal cytoplasmic gout (5.77% in the ER and 10.50% in the ENR). There was a statistically significant difference in the right testicular volumes (13.07cm3 in the RE and 3.96cm3 in the ENR) and left (12.93cm3 in the RE and 3.38cm3 in the ENR) between seasons (p <0.05), with a firm consistency in ER and flaccid in ENR. The mean osmolarity values ​​of seminal plasma were 352.80m Osmol. There was no difference in the levels of fecal steroidal metabolites of testosterone (MFT) and corticosteroids (MFC) between stations. The results obtained allow us to conclude that the semen collection method by digital manipulation is viable for captive maned wolves without apparent impairment of libido, seminal characteristics and reproductive behavior of the animals; the maned wolf ejaculate presents a fraction of predominantly translucent color, watery appearance, without odor and pH of 6.7; the seasonal influence occurs on semen color, ejaculate volume, total sperm concentration, mobile sperm concentration, percentage of living cells and testicular volume; the MFT detected an increase in the testosterone of the reproductive season, however they also identified androgens of adrenal origin outside the reproductive season, which indicates the need for careful analysis of hormonal data in studies using measurements of fecal steroidal metabolites. This work is original about the influence of seasonality in the reproductive cycle of the maned wolf.