Efeito do exercício físico com predominância aeróbica e anaeróbica sobre a metilação do DNA e perfil de linfócitos em mulheres idosas

• Ano de defesa: 2020
• Autor(a) principal: Machado, Otávio Augusto Soares
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Cruzeiro do Sul; Brasil; Programa de Pós Graduação Interdisciplinar em Ciências da Saúde; Cruzeiro do Sul
• Palavras-chave em Português: Idoso; Inflamação; Exercício; Epigenética; CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/1071

In the last decades the elderly population has grown substantially on all continents. Thus, there is also an increase in the occurrence of numerous pathologies resulting from immune imbalance. On the other hand, regular exercise is associated with a reduction in the incidence of several metabolic, cardiovascular and pulmonary diseases. However, individuals differ entirely in adapting to an exercise program in terms of both modality, cellular and molecular responses modulated by exercise. Therefore, the aim of the present study is to compare the effects of aerobic and / or anaerobic exercise on lymphocyte activation profile and the correlation with the degree of global DNA methylation in older women. This is a cross-sectional study involving 86 women (68 ± 7.5 years), divided into 4 groups according to the exercise: resistance training (RT); water aerobic exercise (H); both (TRTA) or control (CON). To evaluate the functional capability the Timed Up and Go test was used. In order to analyze the profile of the immune system we evaluated the percentage of Th1, Th2, Th17, Treg lymphocytes, Th1 / Th2, Treg / Th17 ratios and the proliferation capacity of these cells. Regarding epigenetic parameters, the global methylation of lymphocyte DNA was analyzed by ELISA. As main results, we observed that when compared to the CON group, all the physical exercise groups performed the functional capability test in a shorter time and showed a higher percentage of global lymphocyte DNA methylation. We also noticed that among the physical exercise groups, the TR and TRTA groups showed higher percentage of global DNA methylation. We did not observe differences between Th lymphocyte profiles. Therefore, we conclude that regular physical exercise significantly improves functional capacity, and TR and TRTA may also increase the percentage of global DNA methylation.