Resposta imune celular e humoral de matrizes pesadas após vacinação com vacina viva ou inativada contra Salmonella Enteritidis

• Ano de defesa: 2014
• Autor(a) principal: Bérto, Letícia dal
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Salmonella Enteritidis; Reposta imune celular e humoral; Citometria de fluxo; Vacina viva; Bacterina; Matrizes pesadas; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2580

Salmonella is an ubiquitous bacterium which, besides causing poultry diseases, has a growing zoonotic impact. This fact has demanded the implementation of diverse control strategies, in which vaccines play a major role. The induction of immune response is one of the items of a broader program for infection control, and it is necessary to understand how immune stimulation responds to the several vaccine programs currently proposed. With the purpose of determining the dynamics of the cells involved in humoral and cellular immune response, 150 broiler breeders (Cobb Slow) were divided into three experimental groups - a control group without vaccine, a group that received the live vaccine, and a group that received the inactivated vaccine - Bacterin - against Salmonella Enteritidis. In order to assess the immune response, flow cytometry was used with specific markers for lymphocytes and macrophages in the total blood, and titration of specific circulating antibodies and IgA in the intestinal mucosa. It has been concluded that the live vaccine against Salmonella Enteritidis stimulates an early response of lymphocytes T and macrophages, important for innate immunity, and essential for an efficient adaptive immune response. Bacterin stimulates the massive presence of macrophages after each vaccination, what is directly related to the intensity of the humoral response for this kind of vaccine. Based on these findings, the application of the live vaccine on the first day of life is highly recommended, once this enables the development of an early immune response, this being of great importance to protect the birds on the first days after hatching, when they are more susceptible to Salmonella infections.