Análises bioquímica, microtomográfica e histomorfometrica do aumento ósseo guiado em calvária de ratos após aplicação intermitente de análogos de paratormônio (PTH)

• Ano de defesa: 2018
• Autor(a) principal: Macedo, Amanda Britto de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Osteogênese; Hormônio paratireóideo; Regeneração óssea; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2089

Introduction: Parathyroid hormone (PTH) is a hormone produced by the parathyroid glands naturally present in the human organism, it is more common in children and adolescents in the growth phase, for that it is also considered one of the growth hormones. It exerts both anabolic and catabolic action on the bone. In animals, the systemic administration of intermittent PTH exerts an anabolic effect on the cortical and medullary bone, increasing the density and hardness of the bone. Proposition: The main of this research were to perform serum calcium analysis as well as to verify by means of microtomographic and histomorphometric analysis the presence of bone matrix deposition guided in critical defects treated with autograft in calvaria of rats after intraperitoneal supplementation with exogenous parathormone analogue. Materials and methods: For the design of this study, 40 male Wistar Albinus rats were used, which were allocated in 2 groups: Control Group (C) and PTH Group; each group was subdivided in other 2 subgroups for euthanasia at 15 and 60 days of postoperative. All specimens underwent the same surgical procedure, with two critical defects of 5mm diameter with trephine in the parietal bones, one for the removal of bone for autogenous graft and the other for the placement of this particulate bone and assessment of bone augmentation. Both PTH and saline for control were applied once a week from 7 days before surgery until the day of euthanasia. Results: The values obtained were statistically analyzed using non-parametric Kruskal-Wallis and Mann-Whitney tests, with significance of 95% (p <0.05). The results found in this study revealed that, in biochemical aspects, quantification of alkaline phosphatase was statistically similar in all groups, and although serum calcium levels were similar, the basal levels of the ion in the PTH group were above the normocalcemic line. For the PTH group in both tomographic and histological aspects, the presence of mineral matrix was similar to the control group both in 15 and in 60 days. Although there was no statistically significant difference, it was observed that there was a bone increase in all groups. Conclusion: There were no differences in the results of the autograft autograft treatment and PTH enriched autograft to obtain a deposit of mineral matrix for height gain, but the bone increase observed in all groups suggests that the use of the resin helmet with the autograft had influenced in the tissue regeneration of the critical defect.