Análise da expressão gênica da leptina no tecido mamário: tumoral e não tumoral

• Ano de defesa: 2015
• Autor(a) principal: Grenteski, Jana
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Mamas - Câncer; Obesidade; Leptina; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2590

Breast cancer is the second most common cancer worldwide and the most frequent among women. It is considered as a group of different diseases that affect the same organ, originating from the same structure but with different histopathological characteristics, risk factors and responses to systemic therapies. Obesity is associated with an increase in incidence, but the causes and mechanisms of these relationships are unknown. Leptin is an adipocytokine that plays an important role in tumor development and progression of cancer, in particular breast cancer. The association among leptin, obesity and progression of breast cancer has been suggested. But the causes and mechanisms of these relationships are unknown as well. Contradictory data on the angiogenic actions of leptin are being studied, however, some authors suggest that the pro-angiogenic effects of leptin may play a role in cancer. Thus, this study aimed to evaluate the transcriptional expression of the LEP gene, which encodes leptin in an attempt to elucidate the expression of increased plasma mechanism described in the literature. Twenty-six samples were used for mammary tissue thirteen tumor and thirteen non-tumor, and gene expression analysis performed by PCR in real time. The analysis showed a 8.67 more expression in non-tumor tissue, demonstrating not correspond with the literature data. The data generated were further analyzed for hormone receptor, type and tumor size and age of the patients (pre and post menopause), demonstrating the latter a trend to higher leptin expression in tumor tissue. A larger sample and the correlation data as the presence or absence of obesity for example, are needed to better conclusions, but these initial data suggest that there may be a strong posttranscrional regulation in non-tumor tissue.