Envolvimento do inflamassoma NLRP3 no comportamento relacionado à esquizofrenia em animais jovens expostos a ativação imune materna

• Ano de defesa: 2018
• Autor(a) principal: Ventura, Letícia
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Ativação imune materna; Inflamassoma NLRP3; Esquizofrenia; Desordens psiquiátricas
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/15187

Introduction: In the course of brain development, all changes, such as maternal or insecticidal or inflammatory exposure, may compromise the development of fetal brain function. Maternal immune activation (MAI) may be a shared pathway, endangering the development of psychiatric disorders such as schizophrenia, where it is a psychiatric disorder related to neurodevelopment. Diseases involving the central nervous system (CNS) neuroinflammatory framework, with the involvement of proinflammatory cytokines such as interleukin 1β (IL-1β), which becomes mature through the activation of the molecular complex of the NLRP3 inflammation, being activated by lipopolysaccharides ( LPS) Objective: To evaluate the involvement of NLRP3 non-schizophrenia-related inflamma- toma in young animals exposed to maternal immune activation. Methods: The animals were mated for 24 hours, and after, were evaluated or not of a vaginal. On the 15th gestational day, as the probes received an injection of LPS for the experimental group and PBS for the control of the induction of the AIM model. The day of birth of the animals was defined as P0, when they completed 7 and 14 days postnatal, were submitted to MIA and dissected the brain for biochemical analysis of the expression of the inflammatory complex NLRP3 and levels of IL-1β, as well as the Os animals that completed 45 days and were submitted to behavioral tests of locomotion, social interaction and stereotyped and later biochemical analysis of the same parameters, through the use of Western Blot and ELISA, respectively. Results: Young animals, from the prenatal exposure to the LPS, the schizophrenic type behavior at 45 days of age in the locomotion variables, with the increase in the number of crosses, social interaction through the increase in latency, number and the total contact time and the increase in the number of stereotyped movements. In the biochemical analysis, the results of the tests were increased in the activation of the inflammation complex on days 7, 14 and 45 of life, as well as levels of IL-1β were also raised in the day, remaining high in 14 and 45. Conclusion: The data observed in this study show that the activation is related to the communication of a squamophrenic with communication characteristics and altered locomotives. These evidences may be based on studies between neuroinflammation and psychiatric disorders.