The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos

Bibliographic Details
Main Author: Pizol, José Vitor
Publication Date: 2020
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/21503
Summary: Mestrado em Engenharia Zootécnica - Produção Animal / Instituto Superior de Agronomia / Faculdade de Medicina Veterinária. Universidade de Lisboa
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spelling The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinosrockrosecondensed tanninswool fibrewool proteomicsWhite MerinoMestrado em Engenharia Zootécnica - Produção Animal / Instituto Superior de Agronomia / Faculdade de Medicina Veterinária. Universidade de LisboaThis study was carried out with the objective of evaluating the effect of feeding extract of rockrose (Cistus ladanifer L.), a perennial shrub commonly found in the marginal regions of Portugal and rich in condensed tannins (CT), and its effect on the wool proteome of Portuguese White Merinos. Twenty-four Portuguese White Merinos lambs were fed a diet restricted in protein and composed of grass hay (15%) and concentrate (85%), where they were distributed in three experimental groups: control group, composed of 16% crude protein (CP); diet restricted to 12% crude protein (RP) and diet restricted to 12% crude protein and treated with 1.5% rockrose extract (RPCT). After the 5-week experimental period, samples of wool present in an area of approximately 100cm² in the anterior region of the head were collected from each animal and used for subsequent analysis of proteomics based on liquid chromatography - mass spectrometry (LC-MS). The proteomic method identified 110 proteins in the wool fibre. Of this total, 4 showed significance (P<0.05) and could be classified as structural proteins, with three Type I keratins (Keratin 36, Keratin Type I and Keratin 10) and one Keratin Associated Protein (KAP9.2). The differential accumulation results showed that the RPCT group decreased the expression of these proteins in relation to the Control and RP groups. Except for the K36 protein, which had a 19% higher expression for the RPCT group compared to the other two groups. We conclude that the rockrose extract has a minor effect on the wool proteome that affect specifically the parts of the wool fibre where these proteins are located with putative changes in fibre structure and properties. Finally, further microscopy and wool quality traits studies are still needed to confirm and explain these changesISAAlmeida, André MartinhoRepositório da Universidade de LisboaPizol, José Vitor2024-01-01T01:30:25Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/21503TID:203085299engPizol, J.V. - The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos. Lisboa: ISA, 2020, 71 p.info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T16:08:38Zoai:repositorio.ulisboa.pt:10400.5/21503Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:03:58.041827Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
title The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
spellingShingle The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
Pizol, José Vitor
rockrose
condensed tannins
wool fibre
wool proteomics
White Merino
title_short The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
title_full The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
title_fullStr The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
title_full_unstemmed The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
title_sort The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos
author Pizol, José Vitor
author_facet Pizol, José Vitor
author_role author
dc.contributor.none.fl_str_mv Almeida, André Martinho
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pizol, José Vitor
dc.subject.por.fl_str_mv rockrose
condensed tannins
wool fibre
wool proteomics
White Merino
topic rockrose
condensed tannins
wool fibre
wool proteomics
White Merino
description Mestrado em Engenharia Zootécnica - Produção Animal / Instituto Superior de Agronomia / Faculdade de Medicina Veterinária. Universidade de Lisboa
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2024-01-01T01:30:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21503
TID:203085299
url http://hdl.handle.net/10400.5/21503
identifier_str_mv TID:203085299
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pizol, J.V. - The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos. Lisboa: ISA, 2020, 71 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601930248585216

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