In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract

Detalhes bibliográficos
Autor(a) principal: Cunha, Daniela
Data de Publicação: 2024
Outros Autores: Faria-Silva, Catarina, Carvalho, Filomena A., Ascensão, Lia, Simões, Pedro, Carvalheiro, Manuela, Simões, Sandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/178488
Resumo: Publisher Copyright: © 2024 by the authors.
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spelling In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato ExtractPerspectives on Topical Applicationbiogenic synthesisenvironmentally friendly extractionfood waste recoverygreen chemistrygreen tomatosilver nanoparticleswound healingMaterials Science(all)InstrumentationEngineering(all)Process Chemistry and TechnologyComputer Science ApplicationsFluid Flow and Transfer ProcessesSDG 7 - Affordable and Clean EnergySDG 13 - Climate ActionPublisher Copyright: © 2024 by the authors.Biogenic silver nanoparticles (AgNP) are among the fastest-growing nanomaterials due to the simplicity, efficiency, and sustainability of their biosynthesis using phytochemicals as reducing and coating agents. The agro-food industry generates large quantities of organic waste, a renewable source of biomolecules for AgNP biosynthesis. The main objective of this work was to prepare and characterize biogenic AgNP using a green tomato waste extract (TE) obtained by subcritical water extraction. To the best of our knowledge, this is the first report on the use of such an extract in the synthesis of AgNP. The effects of the TE and AgNO3 concentrations, reaction time, pH, and temperature on AgNP physico-chemical characteristics and on in vitro cytotoxicity against HaCaT and THP-1 cells were assessed. Antimicrobial activity was determined in vitro and ex vivo. The wound-healing capability of AgNP was evaluated in vivo in an incisional wound mouse model. The developed AgNP have a Surface Plasmon Resonance (SPR) band between 402 and 406 nm and a size of ±60 nm, and they are negatively charged (−42 mV) and spherical. In vitro and ex vivo studies prove that AgNP do not compromise skin cells and can decrease cutaneous irritation. The AgNP formulated in a gel revealed similar wound-healing properties to a commercial silver-containing topical ointment. Overall, the biogenic synthesis of AgNP employing an extract of agricultural waste obtained by an eco-friendly method is simple and cost-effective and presents the potential for application in skin disease management.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNCunha, DanielaFaria-Silva, CatarinaCarvalho, Filomena A.Ascensão, LiaSimões, PedroCarvalheiro, ManuelaSimões, Sandra2025-02-05T21:16:52Z2024-082024-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/178488eng2076-3417PURE: 101375079https://doi.org/10.3390/app14167309info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-03T01:37:57Zoai:run.unl.pt:10362/178488Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:46:50.955347Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
Perspectives on Topical Application
title In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
spellingShingle In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
Cunha, Daniela
biogenic synthesis
environmentally friendly extraction
food waste recovery
green chemistry
green tomato
silver nanoparticles
wound healing
Materials Science(all)
Instrumentation
Engineering(all)
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes
SDG 7 - Affordable and Clean Energy
SDG 13 - Climate Action
title_short In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
title_full In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
title_fullStr In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
title_full_unstemmed In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
title_sort In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract
author Cunha, Daniela
author_facet Cunha, Daniela
Faria-Silva, Catarina
Carvalho, Filomena A.
Ascensão, Lia
Simões, Pedro
Carvalheiro, Manuela
Simões, Sandra
author_role author
author2 Faria-Silva, Catarina
Carvalho, Filomena A.
Ascensão, Lia
Simões, Pedro
Carvalheiro, Manuela
Simões, Sandra
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Cunha, Daniela
Faria-Silva, Catarina
Carvalho, Filomena A.
Ascensão, Lia
Simões, Pedro
Carvalheiro, Manuela
Simões, Sandra
dc.subject.por.fl_str_mv biogenic synthesis
environmentally friendly extraction
food waste recovery
green chemistry
green tomato
silver nanoparticles
wound healing
Materials Science(all)
Instrumentation
Engineering(all)
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes
SDG 7 - Affordable and Clean Energy
SDG 13 - Climate Action
topic biogenic synthesis
environmentally friendly extraction
food waste recovery
green chemistry
green tomato
silver nanoparticles
wound healing
Materials Science(all)
Instrumentation
Engineering(all)
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes
SDG 7 - Affordable and Clean Energy
SDG 13 - Climate Action
description Publisher Copyright: © 2024 by the authors.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
2024-08-01T00:00:00Z
2025-02-05T21:16:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/178488
url http://hdl.handle.net/10362/178488
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-3417
PURE: 101375079
https://doi.org/10.3390/app14167309
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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