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Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol

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Autor(a) principal: Saldanha, Carlota
Data de Publicação: 2018
Outros Autores: Freitas, Teresa, Herdade, Ana Santos Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10451/34388
Resumo: ©2018 Saldanha C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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spelling Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timololFibrinogenErythrocyteTimololNitric oxideAdenylyl cyclaseGuanylyl cyclase©2018 Saldanha C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedTimolol inhibits erythrocyte membrane acetylcholinesterase (AChE) enzyme activity originates less active enzyme complex forms unable to change the normal values of the nitric oxide (NO) efflux from erythrocytes. The active complex resulting from acetylcholine (ACh) binding to AChE induces higher values of NO efflux. Increased values of NO efflux were also observed in presence of high fibrinogen (Fib) concentration with return to normal levels of NO efflux when ACh is added. Association between NO efflux and erythrocyte deformability values has been evidenced. The objective of this study was to evaluate the effects of high fibrinogen concentration on the erythrocyte NO metabolism and deformability in presence of timolol without and plus adenylyl cyclase or plus guanylyl cyclase inhibitors. Blood samples from healthy donors were divided in aliquots without (control) or with Fib or Fib plus timolol in absence or presence of adenylyl cyclase inhibitor (MDL) or guanylyl cyclase inhibitor (Ly). Erythrocyte deformability (ED), NO efflux, S-nitrosoglutathione (GSNO), nitrite and nitrate values were evaluated by referenced methods. Fib increased the values of NO efflux under the influence of timolol or MDL. Increased values of NO efflux, GSNO, nitrites and nitrates where obtained in presence of Fib plus timolol plus MDL in relation to control to Fib or Fib plus MDL aliquots. Increased nitrites and nitrates values where obtained in presence of Fib plus timolol plus Ly in relation to control, to Fib and Fib plus Ly. Only at high shear stress increased ED values was observed in Fib plus timolol in absence and presence of MDL. In conclusion fibrinogen plus timolol increased ED at high shear stress without or with MDL. Fibrinogen increased NO efflux from erythrocytes and GSNO, nitrite and nitrate levels in presence of timolol plus guanylyl cyclase inhibitor.This work was funded by Fundação para a Ciência e Tecnologia: LISBOA-01-0145-FEDER-007391, project cofunded by FEDER,through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020.OATRepositório da Universidade de LisboaSaldanha, CarlotaFreitas, TeresaHerdade, Ana Santos Silva2018-07-31T10:54:09Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/34388engClin Med Invest, 2018, Volume 3(1): 1-52398-576310.15761/CMI.1000154info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:56:34Zoai:repositorio.ulisboa.pt:10451/34388Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:58:45.247990Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
title Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
spellingShingle Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
Saldanha, Carlota
Fibrinogen
Erythrocyte
Timolol
Nitric oxide
Adenylyl cyclase
Guanylyl cyclase
title_short Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
title_full Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
title_fullStr Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
title_full_unstemmed Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
title_sort Fibrinogen effects of erythrocyte nitric oxide metabolism in presence of timolol
author Saldanha, Carlota
author_facet Saldanha, Carlota
Freitas, Teresa
Herdade, Ana Santos Silva
author_role author
author2 Freitas, Teresa
Herdade, Ana Santos Silva
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Saldanha, Carlota
Freitas, Teresa
Herdade, Ana Santos Silva
dc.subject.por.fl_str_mv Fibrinogen
Erythrocyte
Timolol
Nitric oxide
Adenylyl cyclase
Guanylyl cyclase
topic Fibrinogen
Erythrocyte
Timolol
Nitric oxide
Adenylyl cyclase
Guanylyl cyclase
description ©2018 Saldanha C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
publishDate 2018
dc.date.none.fl_str_mv 2018-07-31T10:54:09Z
2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/34388
url http://hdl.handle.net/10451/34388
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clin Med Invest, 2018, Volume 3(1): 1-5
2398-5763
10.15761/CMI.1000154
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv OAT
publisher.none.fl_str_mv OAT
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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repository.mail.fl_str_mv info@rcaap.pt
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