Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping
| Main Author: | |
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| Publication Date: | 2021 |
| Other Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/127578 |
Summary: | Funding Information: R.M. is supported by Funda??o para a Ci?ncia e a Tecnologia (CEEC position, 2019?2025 investigator). This article is a result of the projects (iNOVA4Health?UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT?Portuguese Foundation for Science and Technology under the projects number PTDC/BTM?TEC/30087/2017 and PTDC/BTM?TEC/30088/2017. B.C.S. is supported by the Cham-palimaud Foundation and the EMBO Installation Grant 3921. Funding Information: Funding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), sup‐ ported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM‐TEC/30087/2017 and PTDC/BTM‐TEC/30088/2017. B.C.S. is supported by the Cham‐ palimaud Foundation and the EMBO Installation Grant 3921. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtypingDiffuse large B‐cell lymphomaDLBCLExosomesExtracellular vesiclesMass spectrometryProteomicsCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistryFunding Information: R.M. is supported by Funda??o para a Ci?ncia e a Tecnologia (CEEC position, 2019?2025 investigator). This article is a result of the projects (iNOVA4Health?UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT?Portuguese Foundation for Science and Technology under the projects number PTDC/BTM?TEC/30087/2017 and PTDC/BTM?TEC/30088/2017. B.C.S. is supported by the Cham-palimaud Foundation and the EMBO Installation Grant 3921. Funding Information: Funding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), sup‐ ported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM‐TEC/30087/2017 and PTDC/BTM‐TEC/30088/2017. B.C.S. is supported by the Cham‐ palimaud Foundation and the EMBO Installation Grant 3921. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The role of extracellular vesicles (EVs) proteome in diffuse large B‐cell lymphoma (DLBCL) pathology, subclassification, and patient screening is unexplored. We analyzed by state‐of‐the‐art mass spectrometry the whole cell and secreted extracellular vesicles (EVs) proteomes of different molecular subtypes of DLBCL, germinal center B cell (GCB subtype), and activated B cell (ABC subtype). After quality control assessment, we compared whole‐cell and secreted EVs proteomes of the two cell‐of‐origin (COO) categories, GCB and ABC subtypes, resulting in 288/1115 significantly differential expressed proteins from the whole‐cell proteome and 228/608 proteins from EVs (adjust p‐value < 0.05/p‐value < 0.05). In our preclinical model system, we demonstrated that the EV prote-ome and the whole‐cell proteome possess the capacity to separate cell lines into ABC and GCB sub-types. KEGG functional analysis and GO enrichment analysis for cellular component, molecular function, and biological process of differential expressed proteins (DEP) between ABC and GCB EVs showed a significant enrichment of pathways involved in immune response function. Other enriched functional categories for DEPs constitute cellular signaling and intracellular trafficking such as B‐cell receptor (BCR), Fc_gamma R‐mediated phagocytosis, ErbB signaling, and endocyto-sis. Our results suggest EVs can be explored as a tool for patient diagnosis, follow‐up, and disease monitoring. Finally, this study proposes novel drug targets based on highly expressed proteins, for which antitumor drugs are available suggesting potential combinatorial therapies for aggressive forms of DLBCL. Data are available via ProteomeXchange with identifier PXD028267.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)iNOVA4Health - pólo NMSRUNCarvalho, Ana SofiaBaeta, HenriqueHenriques, Andreia F.A.Ejtehadifar, MostafaTranfield, Erin M.Sousa, Ana LauraFarinho, AnaSilva, Bruno CostaCabeçadas, JoséGameiro, Paulada Silva, Maria GomesBeck, Hans ChristianMatthiesen, Rune2021-11-11T23:38:53Z2021-10-012021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/127578eng1661-6596PURE: 34467531https://doi.org/10.3390/ijms222011004info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:57:08Zoai:run.unl.pt:10362/127578Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:28:07.851968Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| title |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| spellingShingle |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping Carvalho, Ana Sofia Diffuse large B‐cell lymphoma DLBCL Exosomes Extracellular vesicles Mass spectrometry Proteomics Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry |
| title_short |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| title_full |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| title_fullStr |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| title_full_unstemmed |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| title_sort |
Proteomic landscape of extracellular vesicles for diffuse large b‐cell lymphoma subtyping |
| author |
Carvalho, Ana Sofia |
| author_facet |
Carvalho, Ana Sofia Baeta, Henrique Henriques, Andreia F.A. Ejtehadifar, Mostafa Tranfield, Erin M. Sousa, Ana Laura Farinho, Ana Silva, Bruno Costa Cabeçadas, José Gameiro, Paula da Silva, Maria Gomes Beck, Hans Christian Matthiesen, Rune |
| author_role |
author |
| author2 |
Baeta, Henrique Henriques, Andreia F.A. Ejtehadifar, Mostafa Tranfield, Erin M. Sousa, Ana Laura Farinho, Ana Silva, Bruno Costa Cabeçadas, José Gameiro, Paula da Silva, Maria Gomes Beck, Hans Christian Matthiesen, Rune |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) iNOVA4Health - pólo NMS RUN |
| dc.contributor.author.fl_str_mv |
Carvalho, Ana Sofia Baeta, Henrique Henriques, Andreia F.A. Ejtehadifar, Mostafa Tranfield, Erin M. Sousa, Ana Laura Farinho, Ana Silva, Bruno Costa Cabeçadas, José Gameiro, Paula da Silva, Maria Gomes Beck, Hans Christian Matthiesen, Rune |
| dc.subject.por.fl_str_mv |
Diffuse large B‐cell lymphoma DLBCL Exosomes Extracellular vesicles Mass spectrometry Proteomics Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry |
| topic |
Diffuse large B‐cell lymphoma DLBCL Exosomes Extracellular vesicles Mass spectrometry Proteomics Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry |
| description |
Funding Information: R.M. is supported by Funda??o para a Ci?ncia e a Tecnologia (CEEC position, 2019?2025 investigator). This article is a result of the projects (iNOVA4Health?UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT?Portuguese Foundation for Science and Technology under the projects number PTDC/BTM?TEC/30087/2017 and PTDC/BTM?TEC/30088/2017. B.C.S. is supported by the Cham-palimaud Foundation and the EMBO Installation Grant 3921. Funding Information: Funding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), sup‐ ported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM‐TEC/30087/2017 and PTDC/BTM‐TEC/30088/2017. B.C.S. is supported by the Cham‐ palimaud Foundation and the EMBO Installation Grant 3921. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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2021 |
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2021-11-11T23:38:53Z 2021-10-01 2021-10-01T00:00:00Z |
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