Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
| Main Author: | |
|---|---|
| Publication Date: | 2023 |
| Other Authors: | , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10451/57606 |
Summary: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscleIntravital microscopyLeukostasisInflammationMicrocirculation© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Inflammation is essential in the protection of the organism and wound repair, but in cases of chronic inflammation can also cause microvasculature deterioration. Thus, inflammation monitorization studies are important to test potential therapeutics. The intravital microscopy (IVM) technique monitors leukocyte trafficking in vivo, being a commonly used procedure to report systemic conditions. Although the cremaster muscle, an established protocol for IVM, may affect the hemodynamics because of its surgical preparation, only male animals are used, and longitudinal studies over time are not feasible. Thinking how this impacts future studies, our aim is to understand if the IVM technique can be successfully performed using the ear lobe instead of the cremaster muscle. Elevated IL-1β plasmatic concentrations confirmed the systemic inflammation developed in a diabetic animal model, while the elevated number of adherent and rolling leukocytes in the ear lobe allowed for the same conclusion. Thus, this study demonstrates that albeit its thickness, the ear lobe protocol for IVM is efficient, non-invasive, more reliable, cost-effective and timesaving.The project leading to these results has received funding from “la Caixa” Foundation under the project code HR21-00605, ID 100010434 and FCT, I.P under the agreement LCF/PR/HP21/52310015.MDPIRepositório da Universidade de LisboaMota-Silva, IaraCastanho, Miguel A. R. B.Silva-Herdade, Ana S.2023-05-25T10:55:49Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57606engLife 2023, 13(4), 88710.3390/life130408872075-1729info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:57:31Zoai:repositorio.ulisboa.pt:10451/57606Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:30:15.617848Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| title |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| spellingShingle |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle Mota-Silva, Iara Intravital microscopy Leukostasis Inflammation Microcirculation |
| title_short |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| title_full |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| title_fullStr |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| title_full_unstemmed |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| title_sort |
Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle |
| author |
Mota-Silva, Iara |
| author_facet |
Mota-Silva, Iara Castanho, Miguel A. R. B. Silva-Herdade, Ana S. |
| author_role |
author |
| author2 |
Castanho, Miguel A. R. B. Silva-Herdade, Ana S. |
| author2_role |
author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Mota-Silva, Iara Castanho, Miguel A. R. B. Silva-Herdade, Ana S. |
| dc.subject.por.fl_str_mv |
Intravital microscopy Leukostasis Inflammation Microcirculation |
| topic |
Intravital microscopy Leukostasis Inflammation Microcirculation |
| description |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-05-25T10:55:49Z 2023 2023-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/57606 |
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http://hdl.handle.net/10451/57606 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Life 2023, 13(4), 887 10.3390/life13040887 2075-1729 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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