Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy

Bibliographic Details
Main Author: Silva-Herdade, Ana S.
Publication Date: 2020
Other Authors: Mota-Silva, Iara, Calado, Ângelo, Saldanha, Carlota, Nguyen, Ngan-Ha, Hou, Isabella, Castanho, Miguel A. R. B., Roy, Sayon
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/46681
Summary: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathyMicrocirculationLeukostasisDiabetic retinopathy© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR.MDPIRepositório da Universidade de LisboaSilva-Herdade, Ana S.Mota-Silva, IaraCalado, ÂngeloSaldanha, CarlotaNguyen, Ngan-HaHou, IsabellaCastanho, Miguel A. R. B.Roy, Sayon2021-03-05T12:40:38Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46681engBiomolecules 2020, 10, 158310.3390/biom101115832218-273Xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:29:13Zoai:repositorio.ulisboa.pt:10451/46681Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:13:35.244307Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
title Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
spellingShingle Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
Silva-Herdade, Ana S.
Microcirculation
Leukostasis
Diabetic retinopathy
title_short Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
title_full Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
title_fullStr Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
title_full_unstemmed Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
title_sort Effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues : implications for diabetic retinopathy
author Silva-Herdade, Ana S.
author_facet Silva-Herdade, Ana S.
Mota-Silva, Iara
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel A. R. B.
Roy, Sayon
author_role author
author2 Mota-Silva, Iara
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel A. R. B.
Roy, Sayon
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva-Herdade, Ana S.
Mota-Silva, Iara
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel A. R. B.
Roy, Sayon
dc.subject.por.fl_str_mv Microcirculation
Leukostasis
Diabetic retinopathy
topic Microcirculation
Leukostasis
Diabetic retinopathy
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-03-05T12:40:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/46681
url http://hdl.handle.net/10451/46681
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomolecules 2020, 10, 1583
10.3390/biom10111583
2218-273X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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