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Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures

Bibliographic Details
Main Author: Grilo, Miguel
Publication Date: 2022
Other Authors: Amaro, Guadalupe, Chambel, Lelia, Marques, Carolina S., Marques, Tiago A., Gil, Fátima, Sousa-Santos, Carla, Robalo, Joana I., Oliveira, Manuela
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/24233
Summary: Research Areas: Agriculture ; Veterinary Sciences ; Zoology
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spelling Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measuresEx situAeromonas sppIberochondrostoma lusitanicumVirulenceAntimicrobial resistanceResearch Areas: Agriculture ; Veterinary Sciences ; ZoologyEx situ breeding programs are important conservation tools for endangered freshwater fish. However, developing husbandry techniques that decrease the likelihood of disease, antimicrobial resistance, and virulence determinants acquisition during this process is challenging. In this pilot study, we conducted a captivity experiment with Portuguese nase (Iberochondrostoma lusitanicum), a critically endangered leuciscid species, to investigate the influence of simple protective measures (i.e., material disinfection protocols and animal handling with gloves) on the dynamics of a potential pathogenic genus, Aeromonas, as well as its virulence profiles and antimicrobial resistance signatures. Our findings show that antimicrobial resistance in Aeromonas spp. collected from I. lusitanicum significantly increased during the extent of the assay (5 weeks), with all isolates collected at the end of the study classified as multidrug-resistant. Additionally, humans handling fishes without protective measures were colonized by Aeromonas spp. The use of protective measures suggested a decreasing trend in Aeromonas spp. prevalence in I. lusitanicum, while bacterial isolates displayed significantly lower virulence index values when virulence phenotypical expression was tested at 22 °C. Despite this study representing an initial trial, which needs support from further research, protective measures tested are considered a simple tool to be applied in ex situ breeding programs for aquatic animals worldwide. Furthermore, current results raise concern regarding antimicrobial resistance amplification and zoonotic transmission of Aeromonas spp. in aquatic ex situ programs.MDPIRepositório da Universidade de LisboaGrilo, MiguelAmaro, GuadalupeChambel, LeliaMarques, Carolina S.Marques, Tiago A.Gil, FátimaSousa-Santos, CarlaRobalo, Joana I.Oliveira, Manuela2022-05-04T22:04:07Z2022-02-112022-02-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24233engGrilo ML, Amaro G, Chambel L, Marques CS, Marques TA, Gil F, Sousa-Santos C, Robalo JI, Oliveira M. 2022. Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures. Animals, 12(4):436. Doi 10.3390/ani120404362076-261510.3390/ani12040436info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:24:43Zoai:repositorio.ulisboa.pt:10400.5/24233Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:42:28.657979Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
title Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
spellingShingle Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
Grilo, Miguel
Ex situ
Aeromonas spp
Iberochondrostoma lusitanicum
Virulence
Antimicrobial resistance
title_short Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
title_full Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
title_fullStr Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
title_full_unstemmed Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
title_sort Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures
author Grilo, Miguel
author_facet Grilo, Miguel
Amaro, Guadalupe
Chambel, Lelia
Marques, Carolina S.
Marques, Tiago A.
Gil, Fátima
Sousa-Santos, Carla
Robalo, Joana I.
Oliveira, Manuela
author_role author
author2 Amaro, Guadalupe
Chambel, Lelia
Marques, Carolina S.
Marques, Tiago A.
Gil, Fátima
Sousa-Santos, Carla
Robalo, Joana I.
Oliveira, Manuela
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Grilo, Miguel
Amaro, Guadalupe
Chambel, Lelia
Marques, Carolina S.
Marques, Tiago A.
Gil, Fátima
Sousa-Santos, Carla
Robalo, Joana I.
Oliveira, Manuela
dc.subject.por.fl_str_mv Ex situ
Aeromonas spp
Iberochondrostoma lusitanicum
Virulence
Antimicrobial resistance
topic Ex situ
Aeromonas spp
Iberochondrostoma lusitanicum
Virulence
Antimicrobial resistance
description Research Areas: Agriculture ; Veterinary Sciences ; Zoology
publishDate 2022
dc.date.none.fl_str_mv 2022-05-04T22:04:07Z
2022-02-11
2022-02-11T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24233
url http://hdl.handle.net/10400.5/24233
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Grilo ML, Amaro G, Chambel L, Marques CS, Marques TA, Gil F, Sousa-Santos C, Robalo JI, Oliveira M. 2022. Aeromonas spp. prevalence, virulence, and antimicrobial resistance in an ex situ program for threatened freshwater fish-a pilot study with protective measures. Animals, 12(4):436. Doi 10.3390/ani12040436
2076-2615
10.3390/ani12040436
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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