The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase

Bibliographic Details
Main Author: Ferreira, Delfim
Publication Date: 2022
Other Authors: Barbosa, Ana C. C., Oliveira, Gonçalo P., Catarino, Teresa, Venceslau, Sofia S., Pereira, Inês A. C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/142213
Summary: Funding Information: ACKNOWLEDGMENTS. We thank Christiane Dahl from the University of Bonn for critically reading the manuscript. This work was funded by the Fundac¸ão para a Ciência e Tecnologia (Portugal) through Fellowships PD/BD/135488/2018 (A.C.C.B.), PD/BD/128204/2016 (D.F.), and SFRH/BPD/79823/2011 (S.S.V.); Grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-BQM/29118/2017; and Research unit Molecular and Structural Microbiology (MOSTMICRO-ITQB) (Grants UIDB/04612/ 2020 and UIDP/04612/2020). The European Union’s Horizon 2020 Research and Innovation Program (Grant Agreement No. 810856) is also acknowledged. Publisher Copyright: © 2022 National Academy of Sciences. All rights reserved.
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spelling The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductaseAllosteric activationDissimilatory sulfite reductaseSulfate-reducing bacteriaSulfur disproportionationSulfur metabolismGeneralFunding Information: ACKNOWLEDGMENTS. We thank Christiane Dahl from the University of Bonn for critically reading the manuscript. This work was funded by the Fundac¸ão para a Ciência e Tecnologia (Portugal) through Fellowships PD/BD/135488/2018 (A.C.C.B.), PD/BD/128204/2016 (D.F.), and SFRH/BPD/79823/2011 (S.S.V.); Grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-BQM/29118/2017; and Research unit Molecular and Structural Microbiology (MOSTMICRO-ITQB) (Grants UIDB/04612/ 2020 and UIDP/04612/2020). The European Union’s Horizon 2020 Research and Innovation Program (Grant Agreement No. 810856) is also acknowledged. Publisher Copyright: © 2022 National Academy of Sciences. All rights reserved.Dissimilatory sulfur metabolism was recently shown to be much more widespread among bacteria and archaea than previously believed. One of the key pathways involved is the dsr pathway that is responsible for sulfite reduction in sulfate-, sulfur-, thiosulfate-, and sulfite-reducing organisms, sulfur disproportionators and organosulfonate degraders, or for the production of sulfite in many photo- and chemotrophic sulfur-oxidizing prokaryotes. The key enzyme is DsrAB, the dissimilatory sulfite reductase, but a range of other Dsr proteins is involved, with different gene sets being present in organisms with a reductive or oxidative metabolism. The dsrD gene codes for a small protein of unknown function and has been widely used as a functional marker for reductive or disproportionating sulfur metabolism, although in some cases this has been disputed. Here, we present in vivo and in vitro studies showing that DsrD is a physiological partner of DsrAB and acts as an activator of its sulfite reduction activity. DsrD is expressed in respiratory but not in fermentative conditions and a ΔdsrD deletion strain could be obtained, indicating that its function is not essential. This strain grew less efficiently during sulfate and sulfite reduction. Organisms with the earliest forms of dsrAB lack the dsrD gene, revealing that its activating role arose later in evolution relative to dsrAB.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaFaculdade de Ciências e Tecnologia (FCT)RUNFerreira, DelfimBarbosa, Ana C. C.Oliveira, Gonçalo P.Catarino, TeresaVenceslau, Sofia S.Pereira, Inês A. C.2022-07-20T22:30:01Z2022-01-252022-01-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/142213eng0027-8424PURE: 36698804https://doi.org/10.1073/pnas.2118880119info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:03:40Zoai:run.unl.pt:10362/142213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:34:24.214112Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
title The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
spellingShingle The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
Ferreira, Delfim
Allosteric activation
Dissimilatory sulfite reductase
Sulfate-reducing bacteria
Sulfur disproportionation
Sulfur metabolism
General
title_short The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
title_full The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
title_fullStr The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
title_full_unstemmed The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
title_sort The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
author Ferreira, Delfim
author_facet Ferreira, Delfim
Barbosa, Ana C. C.
Oliveira, Gonçalo P.
Catarino, Teresa
Venceslau, Sofia S.
Pereira, Inês A. C.
author_role author
author2 Barbosa, Ana C. C.
Oliveira, Gonçalo P.
Catarino, Teresa
Venceslau, Sofia S.
Pereira, Inês A. C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DQ - Departamento de Química
Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Ferreira, Delfim
Barbosa, Ana C. C.
Oliveira, Gonçalo P.
Catarino, Teresa
Venceslau, Sofia S.
Pereira, Inês A. C.
dc.subject.por.fl_str_mv Allosteric activation
Dissimilatory sulfite reductase
Sulfate-reducing bacteria
Sulfur disproportionation
Sulfur metabolism
General
topic Allosteric activation
Dissimilatory sulfite reductase
Sulfate-reducing bacteria
Sulfur disproportionation
Sulfur metabolism
General
description Funding Information: ACKNOWLEDGMENTS. We thank Christiane Dahl from the University of Bonn for critically reading the manuscript. This work was funded by the Fundac¸ão para a Ciência e Tecnologia (Portugal) through Fellowships PD/BD/135488/2018 (A.C.C.B.), PD/BD/128204/2016 (D.F.), and SFRH/BPD/79823/2011 (S.S.V.); Grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-BQM/29118/2017; and Research unit Molecular and Structural Microbiology (MOSTMICRO-ITQB) (Grants UIDB/04612/ 2020 and UIDP/04612/2020). The European Union’s Horizon 2020 Research and Innovation Program (Grant Agreement No. 810856) is also acknowledged. Publisher Copyright: © 2022 National Academy of Sciences. All rights reserved.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-20T22:30:01Z
2022-01-25
2022-01-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/142213
url http://hdl.handle.net/10362/142213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0027-8424
PURE: 36698804
https://doi.org/10.1073/pnas.2118880119
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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