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Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma

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Main Author: Gomes, Sílvia
Publication Date: 2019
Other Authors: Cavadas, Bruno, Ferreira, Joana Catarina, Marques, Patrícia Isabel, Monteiro, Catarina, Sucena, Maria, Sousa, Catarina, Vaz Rodrigues, Luís, Teixeira, Gilberto, Pinto, Paula, Tavares de Abreu, Tiago, Bárbara, Cristina, Semedo, Júlio, Mota, Leonor, Carvalho, Ana Sofia, Matthiesen, Rune, Pereira, Luísa, Seixas, Susana
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.1038/s41598-019-49195-w
Summary: We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029).
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spelling Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinomaGeneralSDG 3 - Good Health and Well-beingWe would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029).The lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing approach of 16S ribosomal RNA gene (V3-V6) using a total of 103 bronchoalveaolar lavage fluid samples. Then, identified taxa were used to inspect 1009 cases from The Cancer Genome Atlas and to annotate tumor unmapped RNAseq reads. Microbial diversity was analyzed per cancer subtype, history of cigarette smoking and airflow obstruction, among other clinical data. We show that LC microbiota is enriched in Proteobacteria and more diverse in SCC than ADC, particularly in males and heavier smokers. High frequencies of Proteobacteria were found to discriminate a major cluster, further subdivided into well-defined communities’ associated with either ADC or SCC. Here, a SCC subcluster differing from other cases by a worse survival was correlated with several Enterobacteriaceae. Overall, this study provides first evidence for a correlation between lung microbiota and cancer subtype and for its influence on patient life expectancy.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNGomes, SílviaCavadas, BrunoFerreira, Joana CatarinaMarques, Patrícia IsabelMonteiro, CatarinaSucena, MariaSousa, CatarinaVaz Rodrigues, LuísTeixeira, GilbertoPinto, PaulaTavares de Abreu, TiagoBárbara, CristinaSemedo, JúlioMota, LeonorCarvalho, Ana SofiaMatthiesen, RunePereira, LuísaSeixas, Susana2019-09-20T22:42:11Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-019-49195-weng2045-2322PURE: 14750540http://www.scopus.com/inward/record.url?scp=85071896383&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-019-49195-winfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:41:10Zoai:run.unl.pt:10362/81887Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:12:25.938446Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
title Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
spellingShingle Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
Gomes, Sílvia
General
SDG 3 - Good Health and Well-being
title_short Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
title_full Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
title_fullStr Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
title_full_unstemmed Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
title_sort Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
author Gomes, Sílvia
author_facet Gomes, Sílvia
Cavadas, Bruno
Ferreira, Joana Catarina
Marques, Patrícia Isabel
Monteiro, Catarina
Sucena, Maria
Sousa, Catarina
Vaz Rodrigues, Luís
Teixeira, Gilberto
Pinto, Paula
Tavares de Abreu, Tiago
Bárbara, Cristina
Semedo, Júlio
Mota, Leonor
Carvalho, Ana Sofia
Matthiesen, Rune
Pereira, Luísa
Seixas, Susana
author_role author
author2 Cavadas, Bruno
Ferreira, Joana Catarina
Marques, Patrícia Isabel
Monteiro, Catarina
Sucena, Maria
Sousa, Catarina
Vaz Rodrigues, Luís
Teixeira, Gilberto
Pinto, Paula
Tavares de Abreu, Tiago
Bárbara, Cristina
Semedo, Júlio
Mota, Leonor
Carvalho, Ana Sofia
Matthiesen, Rune
Pereira, Luísa
Seixas, Susana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Gomes, Sílvia
Cavadas, Bruno
Ferreira, Joana Catarina
Marques, Patrícia Isabel
Monteiro, Catarina
Sucena, Maria
Sousa, Catarina
Vaz Rodrigues, Luís
Teixeira, Gilberto
Pinto, Paula
Tavares de Abreu, Tiago
Bárbara, Cristina
Semedo, Júlio
Mota, Leonor
Carvalho, Ana Sofia
Matthiesen, Rune
Pereira, Luísa
Seixas, Susana
dc.subject.por.fl_str_mv General
SDG 3 - Good Health and Well-being
topic General
SDG 3 - Good Health and Well-being
description We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029).
publishDate 2019
dc.date.none.fl_str_mv 2019-09-20T22:42:11Z
2019-12-01
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41598-019-49195-w
url https://doi.org/10.1038/s41598-019-49195-w
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 14750540
http://www.scopus.com/inward/record.url?scp=85071896383&partnerID=8YFLogxK
https://doi.org/10.1038/s41598-019-49195-w
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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