Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10451/39649 |
Resumo: | © The Author(s) 2019 |
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Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma© The Author(s) 2019The lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing approach of 16S ribosomal RNA gene (V3-V6) using a total of 103 bronchoalveaolar lavage fluid samples. Then, identified taxa were used to inspect 1009 cases from The Cancer Genome Atlas and to annotate tumor unmapped RNAseq reads. Microbial diversity was analyzed per cancer subtype, history of cigarette smoking and airflow obstruction, among other clinical data. We show that LC microbiota is enriched in Proteobacteria and more diverse in SCC than ADC, particularly in males and heavier smokers. High frequencies of Proteobacteria were found to discriminate a major cluster, further subdivided into well-defined communities' associated with either ADC or SCC. Here, a SCC subcluster differing from other cases by a worse survival was correlated with several Enterobacteriaceae. Overall, this study provides first evidence for a correlation between lung microbiota and cancer subtype and for its influence on patient life expectancy.IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). Tis work was supported by the Portuguese Foundation for Science and Technology (FCT), fnanced by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/ BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON.2 – O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referência Estratégico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE070162-FEDER-000067, and NORTE-01-0145-FEDER-000029)Springer NatureRepositório da Universidade de LisboaGomes, SílviaCavadas, BrunoFerreira, Joana CatarinaMarques, Patrícia IsabelMonteiro, CatarinaSucena, MariaSousa, CatarinaRodrigues, Luís VazTeixeira, GilbertoPinto, PaulaAbreu, Tiago Tavares deBárbara, CristinaSemedo, JúlioMota, LeonorCarvalho, Ana SofiaMatthiesen, RunePereira, LuísaSeixas, Susana2019-09-30T13:08:21Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/39649engSci Rep. 2019 Sep 6;9(1):128382045-232210.1038/s41598-019-49195-w 1info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:11:26Zoai:repositorio.ulisboa.pt:10451/39649Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:04:57.251774Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| title |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| spellingShingle |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma Gomes, Sílvia |
| title_short |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| title_full |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| title_fullStr |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| title_full_unstemmed |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| title_sort |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
| author |
Gomes, Sílvia |
| author_facet |
Gomes, Sílvia Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Rodrigues, Luís Vaz Teixeira, Gilberto Pinto, Paula Abreu, Tiago Tavares de Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
| author_role |
author |
| author2 |
Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Rodrigues, Luís Vaz Teixeira, Gilberto Pinto, Paula Abreu, Tiago Tavares de Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Gomes, Sílvia Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Rodrigues, Luís Vaz Teixeira, Gilberto Pinto, Paula Abreu, Tiago Tavares de Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
| description |
© The Author(s) 2019 |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-09-30T13:08:21Z 2019 2019-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/39649 |
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http://hdl.handle.net/10451/39649 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Sci Rep. 2019 Sep 6;9(1):12838 2045-2322 10.1038/s41598-019-49195-w 1 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Springer Nature |
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Springer Nature |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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