Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe

Bibliographic Details
Main Author: Silva, J. P. Neves
Publication Date: 2009
Other Authors: Oliveira, M. Elisabete, Coutinho, Paulo J. G.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/9377
Summary: The aim of this study was to get a further knowledge of the interactions between lamellar and non-lamellar forming-phospholipids, as a tool to better understand the membrane dynamics and function. We have studied the behaviour of aqueous mixtures of a cationic lipid (dioctadecyldimethylammonium bromide) and a neutral phospholipid (1-monoolein) in 10 to 150 micro molar total lipid concentration range. The effect of 1-monoolein on the physical properties (microviscosity, hydration and polarity) of the self-organized mixed aggregates was accessed by studying the steady-state fluorescence emission and anisotropy of the dye Nile Red. This information was combined with 90º Static Light Scattering assays in order to detect any structural variations in these vesicle systems. The results were interpreted by the coexistence of vesicular structures and other aggregates probably with a bicontinuous nature.
id RCAP_a33488361baf19e999ee99cbd736cc1c
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/9377
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probeDODABMonooleinNile RedPolarityHydrationScience & TechnologyThe aim of this study was to get a further knowledge of the interactions between lamellar and non-lamellar forming-phospholipids, as a tool to better understand the membrane dynamics and function. We have studied the behaviour of aqueous mixtures of a cationic lipid (dioctadecyldimethylammonium bromide) and a neutral phospholipid (1-monoolein) in 10 to 150 micro molar total lipid concentration range. The effect of 1-monoolein on the physical properties (microviscosity, hydration and polarity) of the self-organized mixed aggregates was accessed by studying the steady-state fluorescence emission and anisotropy of the dye Nile Red. This information was combined with 90º Static Light Scattering assays in order to detect any structural variations in these vesicle systems. The results were interpreted by the coexistence of vesicular structures and other aggregates probably with a bicontinuous nature.The authors would like to thank to FCT-Portugal for funding through Centro de Fisica of University of Minho and through project PTDC/QUI/69795/2006.ElsevierUniversidade do MinhoSilva, J. P. NevesOliveira, M. ElisabeteCoutinho, Paulo J. G.2009-03-052009-03-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/9377engSilva, J. P. N., Oliveira, M. E. C. D. R., & Coutinho, P. J. G. (2009). Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe. Journal of Photochemistry and Photobiology A: Chemistry, 203(1), 32-39. doi: https://doi.org/10.1016/j.jphotochem.2008.12.0161010-603010.1016/j.jphotochem.2008.12.016https://www.sciencedirect.com/science/article/pii/S101060300800511Xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:29:14Zoai:repositorium.sdum.uminho.pt:1822/9377Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:14:31.374261Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
title Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
spellingShingle Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
Silva, J. P. Neves
DODAB
Monoolein
Nile Red
Polarity
Hydration
Science & Technology
title_short Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
title_full Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
title_fullStr Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
title_full_unstemmed Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
title_sort Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe
author Silva, J. P. Neves
author_facet Silva, J. P. Neves
Oliveira, M. Elisabete
Coutinho, Paulo J. G.
author_role author
author2 Oliveira, M. Elisabete
Coutinho, Paulo J. G.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, J. P. Neves
Oliveira, M. Elisabete
Coutinho, Paulo J. G.
dc.subject.por.fl_str_mv DODAB
Monoolein
Nile Red
Polarity
Hydration
Science & Technology
topic DODAB
Monoolein
Nile Red
Polarity
Hydration
Science & Technology
description The aim of this study was to get a further knowledge of the interactions between lamellar and non-lamellar forming-phospholipids, as a tool to better understand the membrane dynamics and function. We have studied the behaviour of aqueous mixtures of a cationic lipid (dioctadecyldimethylammonium bromide) and a neutral phospholipid (1-monoolein) in 10 to 150 micro molar total lipid concentration range. The effect of 1-monoolein on the physical properties (microviscosity, hydration and polarity) of the self-organized mixed aggregates was accessed by studying the steady-state fluorescence emission and anisotropy of the dye Nile Red. This information was combined with 90º Static Light Scattering assays in order to detect any structural variations in these vesicle systems. The results were interpreted by the coexistence of vesicular structures and other aggregates probably with a bicontinuous nature.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-05
2009-03-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/9377
url https://hdl.handle.net/1822/9377
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, J. P. N., Oliveira, M. E. C. D. R., & Coutinho, P. J. G. (2009). Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvatochromic and anisotropy fluorescent probe. Journal of Photochemistry and Photobiology A: Chemistry, 203(1), 32-39. doi: https://doi.org/10.1016/j.jphotochem.2008.12.016
1010-6030
10.1016/j.jphotochem.2008.12.016
https://www.sciencedirect.com/science/article/pii/S101060300800511X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595189493497856

Similar Items