Monoolein as helper lipid for non-viral transfection in mammals

Bibliographic Details
Main Author: Oliveira, Ana Cristina Norberto Gonçalves
Publication Date: 2010
Other Authors: Silva, J. P. Neves, Coutinho, Paulo J. G., Gomes, A. A., Coutinho, O. P., Oliveira, M. Elisabete
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/14095
Summary: Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.
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spelling Monoolein as helper lipid for non-viral transfection in mammalsLipoplexesTransfectionDODABMonooleinScience & TechnologyLipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.The Portuguese Foundation for Science and Technology (FCT) for the financial support to the Center of Physics and Center of Molecular & Environmental Biology and funding through projects PTDC/QUI/69795/2006 and SFRH/BD/46968/2009 are acknowledged.Elsevier Science BVUniversidade do MinhoOliveira, Ana Cristina Norberto GonçalvesSilva, J. P. NevesCoutinho, Paulo J. G.Gomes, A. A.Coutinho, O. P.Oliveira, M. Elisabete20102010-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/14095eng0168-365910.1016/j.jconrel.2010.07.067info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T05:14:40Zoai:repositorium.sdum.uminho.pt:1822/14095Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:16:34.967457Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Monoolein as helper lipid for non-viral transfection in mammals
title Monoolein as helper lipid for non-viral transfection in mammals
spellingShingle Monoolein as helper lipid for non-viral transfection in mammals
Oliveira, Ana Cristina Norberto Gonçalves
Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
title_short Monoolein as helper lipid for non-viral transfection in mammals
title_full Monoolein as helper lipid for non-viral transfection in mammals
title_fullStr Monoolein as helper lipid for non-viral transfection in mammals
title_full_unstemmed Monoolein as helper lipid for non-viral transfection in mammals
title_sort Monoolein as helper lipid for non-viral transfection in mammals
author Oliveira, Ana Cristina Norberto Gonçalves
author_facet Oliveira, Ana Cristina Norberto Gonçalves
Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
author_role author
author2 Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Ana Cristina Norberto Gonçalves
Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
dc.subject.por.fl_str_mv Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
topic Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
description Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/14095
url https://hdl.handle.net/1822/14095
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0168-3659
10.1016/j.jconrel.2010.07.067
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science BV
publisher.none.fl_str_mv Elsevier Science BV
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595845566529536

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