Development and characterization of osteogenic cell sheets in an in vivo model

Bibliographic Details
Main Author: Pirraco, R. P.
Publication Date: 2008
Other Authors: Yamato, M., Marques, A. P., Reis, R. L., Okano, T.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/59215
Summary: [Excerpt] Despite some successes in the tissue engineering field its evolution seems to be tampered by limitations such as cell sourcing and the lack of adequate scaffolds to support cell growth and differentiation. The use of stem cells combined with cell sheet engineering technology seems a promising way to overcome these limitations. In this work bone marrow cells were flushed from 3 weeks old Wistar rat femurs and cultured in basal DMEM medium until subconfluence. Cells were then transferred to thermo-responsive dishes (3 x10⁵ cells/dish) and cultured for 3 weeks in osteogenic medium. [...]
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spelling Development and characterization of osteogenic cell sheets in an in vivo modelCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialScience & Technology[Excerpt] Despite some successes in the tissue engineering field its evolution seems to be tampered by limitations such as cell sourcing and the lack of adequate scaffolds to support cell growth and differentiation. The use of stem cells combined with cell sheet engineering technology seems a promising way to overcome these limitations. In this work bone marrow cells were flushed from 3 weeks old Wistar rat femurs and cultured in basal DMEM medium until subconfluence. Cells were then transferred to thermo-responsive dishes (3 x10⁵ cells/dish) and cultured for 3 weeks in osteogenic medium. [...]info:eu-repo/semantics/publishedVersionMary Ann Liebert Inc.Universidade do MinhoPirraco, R. P.Yamato, M.Marques, A. P.Reis, R. L.Okano, T.20082008-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/59215engPirraco, R. P., Yamato, M., Marques, A. P., et. al. (2008, May). Development and characterization of osteogenic cell sheets in an in vivo model. In TISSUE ENGINEERING PART A (Vol. 14, No. 5, pp. 720-720). 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA: MARY ANN LIEBERT INC.1937-3341info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:36:18Zoai:repositorium.sdum.uminho.pt:1822/59215Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:58:26.233596Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development and characterization of osteogenic cell sheets in an in vivo model
title Development and characterization of osteogenic cell sheets in an in vivo model
spellingShingle Development and characterization of osteogenic cell sheets in an in vivo model
Pirraco, R. P.
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
title_short Development and characterization of osteogenic cell sheets in an in vivo model
title_full Development and characterization of osteogenic cell sheets in an in vivo model
title_fullStr Development and characterization of osteogenic cell sheets in an in vivo model
title_full_unstemmed Development and characterization of osteogenic cell sheets in an in vivo model
title_sort Development and characterization of osteogenic cell sheets in an in vivo model
author Pirraco, R. P.
author_facet Pirraco, R. P.
Yamato, M.
Marques, A. P.
Reis, R. L.
Okano, T.
author_role author
author2 Yamato, M.
Marques, A. P.
Reis, R. L.
Okano, T.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pirraco, R. P.
Yamato, M.
Marques, A. P.
Reis, R. L.
Okano, T.
dc.subject.por.fl_str_mv Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
topic Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
description [Excerpt] Despite some successes in the tissue engineering field its evolution seems to be tampered by limitations such as cell sourcing and the lack of adequate scaffolds to support cell growth and differentiation. The use of stem cells combined with cell sheet engineering technology seems a promising way to overcome these limitations. In this work bone marrow cells were flushed from 3 weeks old Wistar rat femurs and cultured in basal DMEM medium until subconfluence. Cells were then transferred to thermo-responsive dishes (3 x10⁵ cells/dish) and cultured for 3 weeks in osteogenic medium. [...]
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59215
url http://hdl.handle.net/1822/59215
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pirraco, R. P., Yamato, M., Marques, A. P., et. al. (2008, May). Development and characterization of osteogenic cell sheets in an in vivo model. In TISSUE ENGINEERING PART A (Vol. 14, No. 5, pp. 720-720). 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA: MARY ANN LIEBERT INC.
1937-3341
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc.
publisher.none.fl_str_mv Mary Ann Liebert Inc.
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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