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Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies

Detalhes bibliográficos
Autor(a) principal: Saraiva, Diana P.
Data de Publicação: 2020
Outros Autores: Matias, Ana T., Braga, Sofia, Jacinto, António, Cabral, M. Guadalupe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/105760
Resumo: Funding: This work was supported by Tagus Tank Consortium Projects in Medicine Award; Terry Fox 2019 Research Grant from Liga Portuguesa Contra o Cancro; Fundação para a Ciência e Tecnologia (PD/BD/114023/2015 and PD/BD/114053/2015) and iNOVA4Health (UIDB/04462/2020 and DAI/2019/46).
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spelling Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies3D culturebreast cancerdrug screeningimmunotherapyspheroidstumor immune microenvironmentOncologyCancer ResearchSDG 3 - Good Health and Well-beingFunding: This work was supported by Tagus Tank Consortium Projects in Medicine Award; Terry Fox 2019 Research Grant from Liga Portuguesa Contra o Cancro; Fundação para a Ciência e Tecnologia (PD/BD/114023/2015 and PD/BD/114053/2015) and iNOVA4Health (UIDB/04462/2020 and DAI/2019/46).3D cell culture including different cell types, such as immune cells, is a representative platform that mimics the tumor microenvironment. Here we disclose an easy-to-handle 3D co-culture protocol using a scaffold-free technique with the breast cancer cell line MDA-MB-231 and breast cancer patient-derived immune cells from peripheral blood. The method presented is simple, less time-consuming and less expensive when compared to other 3D techniques. Additionally, this is an optimized protocol for the establishment of a 3D system for this cell line, which is normally seen as challenging to spontaneously form spheroids. The addition of patient-derived immune cells to the cancer cells' spheroid allows the study of the crosstalk between both cell types, as well as the assessment of individual therapeutic approaches to intensify the antitumor immune response. In fact, with this model, we observed that patients' immune cells exhibit a wide range of antitumor responses and we further demonstrated that it is possible to manipulate the less effective ones with a canonical stimulus, as a proof-of-concept, in order to improve their ability to lower the viability of tumor cells. Therefore, this platform could be applied for a personalized immune-based drug screening, with results after a maximum of 10 days of culture, in order to develop more tailored breast cancer treatments and ameliorate patients' survival rate.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNSaraiva, Diana P.Matias, Ana T.Braga, SofiaJacinto, AntónioCabral, M. Guadalupe2020-10-17T00:19:27Z2020-08-272020-08-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/105760engPURE: 19929079https://doi.org/10.3389/fonc.2020.01543info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:48:11Zoai:run.unl.pt:10362/105760Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:19:29.583823Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
title Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
spellingShingle Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
Saraiva, Diana P.
3D culture
breast cancer
drug screening
immunotherapy
spheroids
tumor immune microenvironment
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
title_short Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
title_full Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
title_fullStr Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
title_full_unstemmed Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
title_sort Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
author Saraiva, Diana P.
author_facet Saraiva, Diana P.
Matias, Ana T.
Braga, Sofia
Jacinto, António
Cabral, M. Guadalupe
author_role author
author2 Matias, Ana T.
Braga, Sofia
Jacinto, António
Cabral, M. Guadalupe
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Saraiva, Diana P.
Matias, Ana T.
Braga, Sofia
Jacinto, António
Cabral, M. Guadalupe
dc.subject.por.fl_str_mv 3D culture
breast cancer
drug screening
immunotherapy
spheroids
tumor immune microenvironment
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
topic 3D culture
breast cancer
drug screening
immunotherapy
spheroids
tumor immune microenvironment
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
description Funding: This work was supported by Tagus Tank Consortium Projects in Medicine Award; Terry Fox 2019 Research Grant from Liga Portuguesa Contra o Cancro; Fundação para a Ciência e Tecnologia (PD/BD/114023/2015 and PD/BD/114053/2015) and iNOVA4Health (UIDB/04462/2020 and DAI/2019/46).
publishDate 2020
dc.date.none.fl_str_mv 2020-10-17T00:19:27Z
2020-08-27
2020-08-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/105760
url http://hdl.handle.net/10362/105760
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 19929079
https://doi.org/10.3389/fonc.2020.01543
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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