25-hydroxycholesterol effect on membrane structure and mechanical properties

Detalhes bibliográficos
Autor(a) principal: Domingues, Marco
Data de Publicação: 2021
Outros Autores: Gomes, Bárbara, Hollmann, Axel, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10451/54333
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling 25-hydroxycholesterol effect on membrane structure and mechanical properties25-hydroxycholesterolAtomic force microscopyCholesterolForce spectroscopySupported lipid bilayers© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses' entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25-hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25-hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)-enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.This work was supported by Fundação para a Ciência e a Tecnologia—Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/BBB-BQB/3494/2014, as well as by the FCT-MCTES program of “stimulus of scientific employment” CEECIND/02961/2017 to MMD.MDPIRepositório da Universidade de LisboaDomingues, MarcoGomes, BárbaraHollmann, AxelSantos, Nuno C.2022-09-06T11:29:37Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54333engInt J Mol Sci. 2021 Mar 4;22(5):25741661-659610.3390/ijms220525741422-0067info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:48:06Zoai:repositorio.ulisboa.pt:10451/54333Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:25:09.535947Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv 25-hydroxycholesterol effect on membrane structure and mechanical properties
title 25-hydroxycholesterol effect on membrane structure and mechanical properties
spellingShingle 25-hydroxycholesterol effect on membrane structure and mechanical properties
Domingues, Marco
25-hydroxycholesterol
Atomic force microscopy
Cholesterol
Force spectroscopy
Supported lipid bilayers
title_short 25-hydroxycholesterol effect on membrane structure and mechanical properties
title_full 25-hydroxycholesterol effect on membrane structure and mechanical properties
title_fullStr 25-hydroxycholesterol effect on membrane structure and mechanical properties
title_full_unstemmed 25-hydroxycholesterol effect on membrane structure and mechanical properties
title_sort 25-hydroxycholesterol effect on membrane structure and mechanical properties
author Domingues, Marco
author_facet Domingues, Marco
Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
author_role author
author2 Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Domingues, Marco
Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
dc.subject.por.fl_str_mv 25-hydroxycholesterol
Atomic force microscopy
Cholesterol
Force spectroscopy
Supported lipid bilayers
topic 25-hydroxycholesterol
Atomic force microscopy
Cholesterol
Force spectroscopy
Supported lipid bilayers
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-09-06T11:29:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54333
url http://hdl.handle.net/10451/54333
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Mol Sci. 2021 Mar 4;22(5):2574
1661-6596
10.3390/ijms22052574
1422-0067
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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