Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol

Bibliographic Details
Main Author: Gomes, Bárbara
Publication Date: 2017
Other Authors: Santos, Nuno C., Porotto, Matteo
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/33755
Summary: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol25-hydroxycholesterolFusionMeasles virusPeptide© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Measles virus (MV) infection is re-emerging, despite the availability of an effective vaccine. The mechanism of MV entry into a target cell relies on coordinated action between the MV hemagglutinin (H) receptor binding protein and the fusion envelope glycoprotein (F) which mediates fusion between the viral and cell membranes. Peptides derived from the C-terminal heptad repeat (HRC) of F can interfere with this process, blocking MV infection. As previously described, biophysical properties of HRC-derived peptides modulate their antiviral potency. In this work, we characterized a MV peptide fusion inhibitor conjugated to 25-hydroxycholesterol (25HC), a cholesterol derivative with intrinsic antiviral activity, and evaluated its interaction with membrane model systems and human blood cells. The peptide (MV–HC) has a 90% inhibitory concentration (IC90) several logs more advantageous than the equivalent peptide bearing a polyethylene glycol (PEG)-cholesterol moiety. In membrane interaction studies, MV–HC shows a preference for pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers and membranes rich in sphingomyelin, and interacts less with POPC:cholesterol membranes. MV–HC tends to self-aggregate in aqueous solution, in a concentration-dependent manner. Our results suggest that increased membrane interaction dynamicity results from 25HC conjugation, with a concomitant increase in peptide antiviral efficacy.This work was supported by Fundação para a Ciência e Tecnologia—Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) grant PTDC/BBB-BQB/3494/2014, and NIH AI119762, AI121349, and NS091263 to M.P. B.G. also acknowledges FCT-MCTES fellowship SPRH/BD/52431/2013.MDPIRepositório da Universidade de LisboaGomes, BárbaraSantos, Nuno C.Porotto, Matteo2018-06-05T14:05:03Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/33755engMolecules 2017, 22, 18691420-304910.3390/molecules22111869info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:55:05Zoai:repositorio.ulisboa.pt:10451/33755Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:57:54.545245Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
title Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
spellingShingle Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
Gomes, Bárbara
25-hydroxycholesterol
Fusion
Measles virus
Peptide
title_short Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
title_full Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
title_fullStr Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
title_full_unstemmed Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
title_sort Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
author Gomes, Bárbara
author_facet Gomes, Bárbara
Santos, Nuno C.
Porotto, Matteo
author_role author
author2 Santos, Nuno C.
Porotto, Matteo
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gomes, Bárbara
Santos, Nuno C.
Porotto, Matteo
dc.subject.por.fl_str_mv 25-hydroxycholesterol
Fusion
Measles virus
Peptide
topic 25-hydroxycholesterol
Fusion
Measles virus
Peptide
description © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-06-05T14:05:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/33755
url http://hdl.handle.net/10451/33755
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecules 2017, 22, 1869
1420-3049
10.3390/molecules22111869
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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