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The mechanism of formate oxidation by metal-dependent formate dehydrogenases

Bibliographic Details
Main Author: Mota, Cristiano S.
Publication Date: 2011
Other Authors: Rivas, Maria G., Brondino, Carlos D., Moura, Isabel, Moura, José J. G., González, Pablo J., Cerqueira, Nuno M. F. S. A.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/8611
Summary: J Biol Inorg Chem (2011) 16:1255–1268 DOI 10.1007/s00775-011-0813-8
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spelling The mechanism of formate oxidation by metal-dependent formate dehydrogenasesFormate dehydrogenaseReaction mechanismMolybdenumTungstenDensity functional theoryJ Biol Inorg Chem (2011) 16:1255–1268 DOI 10.1007/s00775-011-0813-8Metal-dependent formate dehydrogenases (Fdh) from prokaryotic organisms are members of the dimethyl sulfoxide reductase family of mononuclear molybdenum-containing and tungsten-containing enzymes. Fdhs catalyze the oxidation of the formate anion to carbon dioxide in a redox reaction that involves the transfer of two electrons from the substrate to the active site. The active site in the oxidized state comprises a hexacoordinated molybdenum or tungsten ion in a distorted trigonal prismatic geometry. Using this structural model, we calculated the catalytic mechanism of Fdh through density functional theory tools. The simulated mechanism was correlated with the experimental kinetic properties of three different Fdhs isolated from three different Desulfovibrio species. Our studies indicate that the C–H bond break is an event involved in the rate-limiting step of the catalytic cycle. The role in catalysis of conserved amino acid residues involved in metal coordination and near the metal active site is discussed on the basis of experimental and theoretical results.SpringerRUNMota, Cristiano S.Rivas, Maria G.Brondino, Carlos D.Moura, IsabelMoura, José J. G.González, Pablo J.Cerqueira, Nuno M. F. S. A.2013-01-30T10:06:13Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8611eng1432-1327info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:12:17Zoai:run.unl.pt:10362/8611Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:43:12.981729Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The mechanism of formate oxidation by metal-dependent formate dehydrogenases
title The mechanism of formate oxidation by metal-dependent formate dehydrogenases
spellingShingle The mechanism of formate oxidation by metal-dependent formate dehydrogenases
Mota, Cristiano S.
Formate dehydrogenase
Reaction mechanism
Molybdenum
Tungsten
Density functional theory
title_short The mechanism of formate oxidation by metal-dependent formate dehydrogenases
title_full The mechanism of formate oxidation by metal-dependent formate dehydrogenases
title_fullStr The mechanism of formate oxidation by metal-dependent formate dehydrogenases
title_full_unstemmed The mechanism of formate oxidation by metal-dependent formate dehydrogenases
title_sort The mechanism of formate oxidation by metal-dependent formate dehydrogenases
author Mota, Cristiano S.
author_facet Mota, Cristiano S.
Rivas, Maria G.
Brondino, Carlos D.
Moura, Isabel
Moura, José J. G.
González, Pablo J.
Cerqueira, Nuno M. F. S. A.
author_role author
author2 Rivas, Maria G.
Brondino, Carlos D.
Moura, Isabel
Moura, José J. G.
González, Pablo J.
Cerqueira, Nuno M. F. S. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Mota, Cristiano S.
Rivas, Maria G.
Brondino, Carlos D.
Moura, Isabel
Moura, José J. G.
González, Pablo J.
Cerqueira, Nuno M. F. S. A.
dc.subject.por.fl_str_mv Formate dehydrogenase
Reaction mechanism
Molybdenum
Tungsten
Density functional theory
topic Formate dehydrogenase
Reaction mechanism
Molybdenum
Tungsten
Density functional theory
description J Biol Inorg Chem (2011) 16:1255–1268 DOI 10.1007/s00775-011-0813-8
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-01-30T10:06:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8611
url http://hdl.handle.net/10362/8611
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1432-1327
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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