A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions

Bibliographic Details
Main Author: Fonseca, Catarina
Publication Date: 2023
Other Authors: Silvério, Vânia, Barata, David, Giese, Wolfgang, Gerhardt, Holger, Cardoso, Susana, Franco, Claudio
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/59457
Summary: © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditionsMaterials scienceNanoscale devices© The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.The ability of endothelial cells to respond to blood flow is fundamental for the correct formation and maintenance of a functional and hierarchically organized vascular network. Defective flow responses, in particular related to high flow conditions, have been associated with atherosclerosis, stroke, arteriovenous malformations, and neurodegenerative diseases. Yet, the molecular mechanisms involved in high flow response are still poorly understood. Here, we described the development and validation of a 96-wells fluidic system, with interchangeable cell culture and fluidics, to perform high-throughput screenings under laminar high-flow conditions. We demonstrated that endothelial cells in our newly developed 96-wells fluidic system respond to fluid flow-induced shear stress by aligning along the flow direction and increasing the levels of KLF2 and KLF4. We further demonstrate that our 96-wells fluidic system allows for efficient gene knock-down compatible with automated liquid handling for high-throughput screening platforms. Overall, we propose that this modular 96-well fluidic system is an excellent platform to perform genome-wide and/or drug screenings to identify the molecular mechanisms involved in the responses of endothelial cells to high wall shear stress.C.G.F. was supported by a PhD fellowship from the doctoral program Bioengineering: Cellular Therapies and Regenerative Medicine funded by Fundação para a Ciência e Tecnologia (PD/BD/128375/2017). This work was supported by the European Research Council (679368); European Commission (801423); Fondation LeDucq (17CVD03); Fundação para a Ciência e Tecnologia (PTDC/BIA-CEL/32180/2017; CEECIND/04251/2017; UID/05367/2020; FPJ001377 - PTDC/MED-ANM/7695/2020; FPJ001461 - EXPL/MED-ANM/1616/2021; PTDC-FISPLA/31055/2017; and LA/P/0140/2020).Springer NatureRepositório da Universidade de LisboaFonseca, CatarinaSilvério, VâniaBarata, DavidGiese, WolfgangGerhardt, HolgerCardoso, SusanaFranco, Claudio2023-09-25T14:12:02Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/59457engMicrosyst Nanoeng. 2023 Sep 15;9:114.2096-103010.1038/s41378-023-00589-x2055-7434info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:01:39Zoai:repositorio.ulisboa.pt:10451/59457Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:31:54.175712Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
title A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
spellingShingle A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
Fonseca, Catarina
Materials science
Nanoscale devices
title_short A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
title_full A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
title_fullStr A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
title_full_unstemmed A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
title_sort A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
author Fonseca, Catarina
author_facet Fonseca, Catarina
Silvério, Vânia
Barata, David
Giese, Wolfgang
Gerhardt, Holger
Cardoso, Susana
Franco, Claudio
author_role author
author2 Silvério, Vânia
Barata, David
Giese, Wolfgang
Gerhardt, Holger
Cardoso, Susana
Franco, Claudio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fonseca, Catarina
Silvério, Vânia
Barata, David
Giese, Wolfgang
Gerhardt, Holger
Cardoso, Susana
Franco, Claudio
dc.subject.por.fl_str_mv Materials science
Nanoscale devices
topic Materials science
Nanoscale devices
description © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-25T14:12:02Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/59457
url http://hdl.handle.net/10451/59457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microsyst Nanoeng. 2023 Sep 15;9:114.
2096-1030
10.1038/s41378-023-00589-x
2055-7434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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