Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column

• Main Author: Nobre, Clarisse
• Publication Date: 2012
• Other Authors: Teixeira, J. A., Rodrigues, L. R.
• Format: Article
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: https://hdl.handle.net/1822/19928
• topic: Fructooligosaccharides; Prebiotics; Recovery; Activated charcoal column; Science & Technology

In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth.